Abstract
The various horseradish peroxidase (HRP) methods for labeling of neuronal pathways have become well-characterized tools in the armamentarium of the neuroscientist despite the relatively short period during which the methods have evolved. Another stage of methodological approach is now emerging in which the basic HRP method is being combined with other techniques for special applications. To be sure, such procedures are not restricted to combinations in which the HRP method is a necessary component. The Golgi method can be combined with the analysis of experimentally induced degeneration for the study of interneuronal connections (Chapter 12), and double labeling of neuronal cell bodies, using immunofluorescence for neurotransmitter analyses and a fluorescent retrograde tracer for the study of the neurons’ axonal projections, is described in the Appendix (Section VII.C) of this chapter.
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© 1981 Plenum Press, New York
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Steward, O. (1981). Horseradish Peroxidase and Fluorescent Substances and Their Combination with Other Techniques. In: Heimer, L., Robards, M.J. (eds) Neuroanatomical Tract-Tracing Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3189-6_8
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DOI: https://doi.org/10.1007/978-1-4613-3189-6_8
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