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Horseradish Peroxidase

The Basic Procedure

  • Chapter
Neuroanatomical Tract-Tracing Methods

Abstract

Since its introduction in 1971 (Kristensson et al., 1971, LaVail and LaVail, 1972), the use of retrograde axonal transport of macromolecular tracers, particularly horseradish peroxidase (HRP), has proven to be both a reliable and sensitive method for the positive identification of cell bodies giving origin to fiber projections to a given site within the central or peripheral nervous system (for reviews, see LaVail, 1978, and Jones and Hartmann, 1978). The subsequent demonstration that HRP is transported by axons in the anterograde direction (Hansson, 1973; Lynch et al., 1974; Scalia ad Colman, 1974; LaVail and LaVail, 1974; Sotelo and Riche, 1974; Repérant, 1975; Walberg et al., 1976; de Olmos and Heimer, 1977; Robertson, 1977; Streit and Reubi, 1977; Mesulam and Mufson, 1980) and can be readily detected if histochemical processing is optimal extended the utility of this technique even further. Indeed, innovations and improvements in technique as well as the discovery of new tracer molecules (Kuypers et al., 1977; Schwab and Thoenen, 1977) continue to expand the potential applications of macromolecular tracers in unraveling the intricate circuitry of the nervous system.

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Author information

Authors and Affiliations

  1. The Boys Town Institute for Communication Disorders in Children, Omaha, Nebraska, 68131, USA

    W. Bruce Warr

  2. Instituto de Investigación Medica Mercedes y Martin Ferreyra, Cordoba, Argentina

    José S. de Olmos

  3. Department of Neurology, University of Virginia School of Medicine, Charlottesville, Virginia, 22908, USA

    Lennart Heimer

Authors
  1. W. Bruce Warr
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  2. José S. de Olmos
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  3. Lennart Heimer
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Editor information

Editors and Affiliations

  1. Department of Neurology, University of Virginia School of Medicine, Charlottesville, Virginia, 22908, USA

    Lennart Heimer

  2. University of California, Irvine, California, USA

    Martine J. Robards

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© 1981 Plenum Press, New York

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Warr, W.B., de Olmos, J.S., Heimer, L. (1981). Horseradish Peroxidase. In: Heimer, L., Robards, M.J. (eds) Neuroanatomical Tract-Tracing Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3189-6_6

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  • DOI: https://doi.org/10.1007/978-1-4613-3189-6_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3191-9

  • Online ISBN: 978-1-4613-3189-6

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