Abstract
Because implantation is the increasingly intimate interaction of a blastocyst with the endometrium of an intact uterus, there is really no such thing as in vitro implantation. However, trophoblast in vitro continues to differentiate beyond the preimplantation stage, and double organ culture can be used for cell associations. Therefore, it is possible to make models, if not of normal implantation, at least of ectopic implantation. The precise morphological events of implantation are sufficiently different in different groups of manmials to preclude all but the broadest generalizations concerning implantation (Wimsatt, 1975; Schlafke and Enders, 1975). In considering those species that have been studied by in vitro implantation models, attention has been confined to examples in which there is considerable invasion of the endometrium. Many domestic animals and most of the marsupials, in which implantation is more superficial, have been excluded from these studies.
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References
Blandau, R. J., 1971, Culture of guinea pig blastocyst, in: Biology of the Blastocyst ( R. J. Blandau, ed.), pp. 59–69, Univ. of Chicago Press, Chicago.
Chavez, D. J., 1979, Delayed Implantation: Molecular and Cellular Basis, Ph.D. dissertation, Colorado State University, Fort Collins, Color.
Cole, R. J., and Paul, J., 1965, Properties of cultured preimplantation mouse and rabbit embryos, and cell strains derived from them, in: Preimplantation Stages of Pregnancy ( G. E. W. Wolstenholme and M. O’Conner, eds.), pp. 82–112, Academic Press, New York.
Enders, A. C., 1975, The implantation chamber, blastocyst, and blastocyst imprint of the rat: A scanning electron microscope study, Anat Rec. 182: 137.
Enders, A. C., 1976a, Anatomical aspects of implantation, J. Reprod. Fertil. Suppl. 25: 1.
Enders, A. C., 1976b, Cytology of human early implantation,Reprod. 8: 1.
Enders, A. C., and Nelson, D. M., 1973, Pinocytotic activity of the uterus of the rat, Am. J. Anat. 138: 277.
Enders, A. C., and Schlafke, S., 1979, Comparative aspects of blastocyst-endometrial interactions at implantation, in: Maternal Recognition of Pregnancy, Ciba Foundation Symposium 64, pp. 3–32, Elsevier/North- Holland, Amsterdam.
Enders, A. C., Given, R. L., and Schlafke, S., 1978, Differentiation and migration of endoderm in the rat and mouse at implantation, Rec. 190: 65.
Erickson, R., and Pedersen, R. A., 1975,in vitro development of t 6 /t 6 embryos, J. Exp. Zool. 193: 377.
Glass, R. H., Spindle, A. I., and Pedersen, R. A., 1979, Mouse embryo attachment to substratum and interaction of trophoblast with cultured cells, J. Exp. Zool. 208: 327.
Glenister, T. W., 1962, Embryo-endometrial relationships during nidation organ culture, J. Obstet, Gynaecol. Br. Commonw. 69: 809.
Glenister, T. W., 1963, Observations on mammalian blastocysts implanting in organ culture, in: Delayed Implantation (A. C. Enders, ed.),pp. 171–182, Univ. of Chicago Press, Chicago.
Goldstein, L. S., Spindle, A. I., and Pedersen, R. A., 1975, X-Ray sensitivity of the preimplantation mouse embryo in vitro, Radiat. Res. 62: 276.
Grant, P. S., Ljungkvist, I., and Nilsson, O., 1975, The hormonal control and morphology of blastocyst invasion in the mouse uterus in vitro, J. Embryol. Exp. Morphol. 34: 310.
Gwatkin, R. B. L., 1966, Amino acid requirements for attachment and outgrowth of the mouse blastocyst in vitro, J. Cell. Physiol. 68: 335.
Hedlund, K., Nilsson, O., Reinius, S., and Aman, G., 1972, Attachment reaction of the uterine luminal epithelium at implantation: Light and electron microscopy of the hamster, guinea pig, rabbit and mink, J. Reprod. Fertil. 29: 131.
Heyner, S., 1973, Detection of H-2 antigens on the cells of the early mouse embryo. Transplantation 16: 675.
Jenkinson, E. J., 1978, The in vitro blastocyst outgrowth system as a model for the analysis of peri-implantation development, in: Development in Mammals, Vol. 2 ( M. Johnson, ed.), p. 157, North-Holland, Amsterdam.
Jenkinson, E. J., and Searie, R. F., 1977, Cell surface changes on the mouse blastocyst at implantation, Exp. Cell Res. 106: 386.
Jenkinson, E. J., and Wilson, LB., 1973, In vitro studies on the control of trophoblast outgrowth in the mouse, J. Embryol. Exp. Morphol. 30: 21.
Kubo, H., Spindle, A. L, and Pederson, R. A., 1979, Possible involvement of protease in mouse blastocyst implantation in vitro, Biol. Reprod. Suppl. 20: 50A.
McLaren, A., and Hensleigh, H. C., 1975, Culture of mammalian embryos over the implantation period, in: The Early Development of Mammals ( M. Balls and A. E. Wild, eds.), pp 45–60, Cambridge Univ. Press, London.
Mintz, B., 1964, Formation of genetically mosaic embryos and early development of lethal (t 12 / 12 )-normal mosaics, J. Exp.Zool 157: 273.
Owers, N. O., and Blandau, R. J., 1971, Proteolytic activity of the rat and guinea pig blastocyst in vitro, in: Biology of the Blastocyst ( R. J. Blandau, ed.), pp. 207–223, Univ. of Chicago Press, Chicago.
Pedersen, R. A., 1974, Development of lethal yellow mouse embryos in vitro, J. Exp. Zool. 188: 307.
Pederson, R. A., and Spindle, A. L, 1976, Genetic effects of mammalian development during and after implantation, in Embryogenesis in Mammals, Ciba Foundation Symposium 40, pp. 133–154, Elsevier, Amsterdam.
Pedersen, R. A., and Spindle, A. L, 1977, Interference with in vitro development of mouse inner cell mass by 24-hr treatment with 5-bromodeoxyuridine, Differentiation 9: 43.
Revel, J. P., Darr, G., Griepp, E. B., Johnson, R., and Miller, M. M., 1978, Cell movement and intercellular contact, in: The Molecular Basis of Cell-Cell Interaction ( R. A. Lemer and D. Bergsma, eds.), pp. 67–81, Liss, New York.
Rowinski, J., Solter, P., and Koprowski, H., 1975, Mouse embryo development m vitro: Effects of inhibitors of RNA and protein synthesis on blastocyst and post-blastocyst embryos, J. Exp. Zool. 192: 133.
Salomon, D. S., and Sherman, M. I., 1975, Implantation and invasiveness of mouse blastocysts on uterine monolayers, Exp. Cell Res. 90: 261.
Schlafke, S., and Enders, A. C., 1975, Cellular basis of interaction between trophoblast and uterus at implantation, Reprod. 12: 41.
Sherman, M. I., 1978, Implantation of mouse blastocysts in vitro, in: Methods in Mammalian Reproduction ( J. C. Daniel, Jr., ed.), pp. 247–257, Academic Press, New York.
Sherman, M. I., and Salomon, D. S., 1975, The relationships between the early mouse embryo and its environment, in: The Developmental Biology of Reproduction ( C. L. Markert and J. Papaconstantinou, eds.), pp. 277–309, Academic Press, New York.
Sherman, M. I., and Wudl, L. R., 1976, The implanting mouse blastocyst, in: The Cell Surface in Animal Embryogenesis and Development ( G. Poste and G. L. Nicolson, eds.), pp. 81–125, Elsevier/North-Holland, Amsterdam.
Sherman, M. I., and Wudl, L., 1977, T-complex mutations and their effects, in: Concepts in Mammalian Embryogenesis (M. I. Sherman, ed.), pp. 136–234, MTT Press, Cambridge, Mass.
Sherman, M. I., McLaren, A., and Walker, P. M. B., 1972, Mechanism of accumulation of DNA in giant cells of mouse trophoblast, Nature (London) New Biol. 238: 175.
Sherman, M. I., Shalgi, R., Rizzino, A., Sellens, M. H., Gay, S., and Gay, R., 1979, Changes in the surface of the mouse blastocyst at implantation, in: Maternal Recognition of Pregnancy, Ciba Foundation Symposium 64, pp. 33–47, Elsevier/North-Holland, Amsterdam.
Spindle, A. I., and Pedersen, R. A., 1973, Hatching, attachment, and outgrowth of mouse blastocysts in vitro: Fixed nitrogen requirements, J. Exp. Zool. 186: 305.
Strickland, S., Reich, E., and Sherman, M. I., 1976, Plasminogen activator in early embryogenesis: Enzyme production by trophoblast and parietal endoderm. Cell 9: 231.
Surani, M. A. H., 1977, Response of preimplantation rat blastocysts in vitro to extracellular uterine luminal components, serum and hormones, J. Cell Sci. 25: 265.
Wiley, L. M., and Pedersen, R. A., 1978, Morphology of mouse egg cylinder development in vitro: A light and electron microscopic study, J. Exp. Zool. 200: 389.
Wimsatt, W. A., 1975, Some comparative aspects of implantation, Biol. Reprod. 12: 1.
Wordinger, R. J., and Brinster, R. L., 1976, Influence of reduced glucose levels on the in vitro hatching, attachment, and trophoblast outgrowth of the mouse blastocyst. Dev. Biol. 53: 294.
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© 1981 Plenum Press, New York
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Enders, A.C., Chávez, D.J., Schlafke, S. (1981). Comparison of Implantation in Utero and in Vitro. In: Glasser, S.R., Bullock, D.W. (eds) Cellular and Molecular Aspects of Implantation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3180-3_29
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DOI: https://doi.org/10.1007/978-1-4613-3180-3_29
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