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Quantitation of Murine IgE in an Automatic Elisa System

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Microenvironments in the Lymphoid System

Abstract

We describe a two-site micro ELISA system for rapid, automatic and quantitative measurement of total IgE in mouse serum, that is based upon a hybridoma IgE standard and heterologous anti-IgE antibodies. With this method we determined the absolute total IgE concentration in the serum of aging athymic nude mice and their heterozygous litter mates.

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© 1985 Plenum Press, New York

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Savelkoul, H.F.J., Soeting, P.W.C., Benner, R., Radl, J. (1985). Quantitation of Murine IgE in an Automatic Elisa System. In: Klaus, G.G.B. (eds) Microenvironments in the Lymphoid System. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2463-8_92

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  • DOI: https://doi.org/10.1007/978-1-4613-2463-8_92

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9495-5

  • Online ISBN: 978-1-4613-2463-8

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