Abstract
If one asks, which criteria might be mandatory for the opinion on an enzyme complex, the function of which is not yet fully understood, the integral stoichiometry of all components of the isolate must be considered a guideline for preparation and purification besides known enzymatic activities and pertinent spectral properties. Application of this principal chemical rule is especially necessary in those fields of work, where our methods leave doubt, whether what we obtain is a faithful representation of what exists in nature. This is the case with the respiratory enzyme complexes, e.g. mitochondrial cytochrome c oxidase. The solubilisation and purification of this enzyme from the mitochondrial (or bacterial) membrane has in many cases not met the above criterion. The complete proteinchemical description of the so far most complex type of this enzyme, obtained from bovine heart, and its comparison with simpler eucaryotic and procaryotic oxidases defines the structure of integral preparations more precisely and at the same time is a guide to the function.
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References
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Buse, G., Steffens, G.C.M., Biewald, R., Bruch, B., Hensel, S. (1987). Respiratory Complex IV and Cytochrome a,a3 . In: Papa, S., Chance, B., Ernster, L. (eds) Cytochrome Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1941-2_35
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DOI: https://doi.org/10.1007/978-1-4613-1941-2_35
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