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Steroid hormone regulation of cultured breast cancer cells

  • Chapter
Breast Cancer: Cellular and Molecular Biology

Part of the book series: Cancer Treatment and Research ((CTAR,volume 40))

Abstract

Most of the information we have about the regulation of breast tumor growth relates to hormones, especially steroid hormone effects. Such actions are diverse and complex involving direct effects on the tumor cells and indirect effects via other endocrine and non-endocrine organs. Because of this complexity, it is extremely difficult to interpret in vivo data at the required level of cellular and molecular organization so cell culture systems have to be extensively used. Despite the general dogma that ‘cancer cells have escaped from growth control’, establishment of cultures from primary breast tumors has been difficult, more difficult in fact than with normal mammary cells [1]. Furthermore, the establishment of meaningful hormonal regulation of such cultures has been elusive. This has led many workers, ourselves included, to use established cell lines to answer the question of how do steroids affect mammary tumor cell growth? Cell lines clearly have the advantage of relative cellular homogeneity, reproducibility from one experiment to another, ease of experimental manipulation and an approximately defined medium in which growth can be studied. The word ‘approximately’ is used because, although serum free conditions have been used for looking at short to medium-term effects of steroids [2–4], serum is present in the majority of such experiments and is always used for long-term maintenance of such cultures.

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  1. Philippa D. Darbre
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  2. Roger J. B. King
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  1. National Cancer Institute, USA

    Marc E. Lippman  & Robert B. Dickson  & 

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Darbre, P.D., King, R.J.B. (1988). Steroid hormone regulation of cultured breast cancer cells. In: Lippman, M.E., Dickson, R.B. (eds) Breast Cancer: Cellular and Molecular Biology. Cancer Treatment and Research, vol 40. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1733-3_15

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