Abstract
Nodules are independent, spherical, dense cell clusters which form a cohesive unit and display a consistent internal cell/tissue differentiation. At a minimum, three cell types (meristematic cells, plastid-dense parenchyma, and vascular elements) and two cell layers (epidermal and internal cortex/vascular) can be distinguished in nodules of poplar. Although nodules have been randomly observed by many researchers working with a myriad of plant species, they most commonly are seen in cultures of woody plant species being differentiated from dedifferentiated cells. We have developed liquid culture systems in which nodules are the predominant structures. Such cultures grow via nodule enlargement (a three-stage process) and nodule multiplication (via two general pathways). In general, nodules display a high capacity for plant/organ regeneration via organogenesis. The nodular developmental pathway parallels that of the embryogenic developmental pathway; a theoretical comparison of the two pathways as bridges between totipotency and competence is discussed. Although the research is still very preliminary, nodule cultures have apparent applications in regeneration strategies, automated micropropagation, and in vitro phytochemical production.
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McCown, B.H., Zeldin, E.L., Pinkalla, H.A., Dedolph, R.R. (1988). Nodule Culture: A Developmental Pathway with High Potential for Regeneration, Automated Micropropagation, and Plant Metabolite Production from Woody Plants. In: Hanover, J.W., Keathley, D.E., Wilson, C.M., Kuny, G. (eds) Genetic Manipulation of Woody Plants. Basic Life Sciences, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1661-9_9
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DOI: https://doi.org/10.1007/978-1-4613-1661-9_9
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