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Studies on Crystals of Intact Bacterial Ribosomal Particles

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Structure, Function, and Genetics of Ribosomes

Part of the book series: Springer Series in Molecular Biology ((SSMOL))

Abstract

A reliable molecular model for ribosomes is essential for the understanding of their role in protein biosynthesis. In parallel to extensive chemical, physical, immunological, and genetic studies, we have extended our efforts toward the use of X-ray crystallography and three-dimensional reconstruction of electron micrographs. These techniques are very powerful since they offer direct structural information. Their application depends on the availability of crystalline materials.

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References

  • Abdel-Meguid, S.S., Moore, P.B., Steitz, TA. (1983). Crystallization of a ribonuclase-resistant fragment of E. coli 5S ribosomal RNA and its complex with protein L25, J. Mol. Biol 171: 207–215.

    CAS  Google Scholar 

  • Appelt, K., Dijk, J., Epp, O. (1979). The crystallization of protein BL17 from the 50 S ribosomal subunit of B. stearothermophilus. FEBS Lett. 103: 66–70.

    Article  PubMed  CAS  Google Scholar 

  • Appelt, K., Dijk, J., Reinhardt, R., Sanhuesa, S., White, S.W., Wilson, K.S., Yonath, A. (1981). The crystallization of ribosomal proteins from the 50S subunit of the E. coli and B. stearothermophilus ribosome. J. Biol. Chem 256: 11787–11790.

    CAS  Google Scholar 

  • Appelt, K., Dijk, J., White, S.T., Wilson, K.S., Bartels, K. (1983 a). Proteins of the B. stearothermophilus ribosome: A low resolution crystal analysis of protein L30. FEBS Lett. 160: 72–74.

    Google Scholar 

  • Appelt, K., Dijk, J., White, S.T., Wilson, K.S. (1983b). Proteins of the B. stearother-mophilus ribosome: crystallization of protein L6. FEBS Lett. 160: 75–77.

    Article  PubMed  CAS  Google Scholar 

  • Appelt, K., White, S.T., Wilson, K.S. (1983 c). Proteins of the B. stearothermophilus ribosome: crystallization of proteins L30 and S5. J. Biol. Chem 258: 13328–13340.

    Google Scholar 

  • Appelt, K., Tanaka, I., White, S.T., Wilson, K.S. (1984). Proteins of the B. stearothermophilus ribosome: the crystal structure of L6 at 6 A resolution. FEBS Lett. 165: 43–45.

    Article  CAS  Google Scholar 

  • Arad, T., Leonard, K.R., Wittmann, H.G., Yonath, A. (1984a). Two-dimensional crystalline sheets of B. stearothermophilus ribosomal particles. EMBO J. 3: 127–131.

    PubMed  CAS  Google Scholar 

  • Arad, T., Leonard, K.R., Wittmann, H.G., Yonath, A. (1984b). Three-dimensional reconstruction studies of 50 S ribosomal particles. Fourth International Congress on Electron Microscopy, p. 1459.

    Google Scholar 

  • Barbieri, M. (1979). Ribosome crystallization in homogenates and cell extracts of chick embryos. J. Supramol. Struct 10: 349–357.

    Article  PubMed  CAS  Google Scholar 

  • Clark, M.W., Hammons, M., Langer, J.A., Lake, J.A. (1979). Helical arrays of E. coli small ribosomal subunits produced in vitro. J. Mol. Biol 135: 507–512.

    CAS  Google Scholar 

  • Clark, M.W., Leonard, K., Lake, J.A. (1982). Ribosomal crystalline arrays of large subunits from E. coli. Science 216: 999–1001.

    CAS  Google Scholar 

  • Chambliss, G., Craven, G.R., Davies, J., Davis, K., Kahan, L., Nomura, M. eds. (1980). Ribosomes: structure, function and genetics. University Park Press, Baltimore.

    Google Scholar 

  • Finch, J.T., Lutter, L.C., Rhodes, D., Brown, R.S., Rushton, B., Levitt, M., Klug, A. (1977). Structure of the nucleosome core particles of chromatin. Nature 269: 29–41.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, J.E., Hollingshead, C. (1981). Crystallographic studies of cowpea mosaic virus by electron microscopy and X-ray diffraction. J. Ultrastruct. Res 74: 223–231.

    Article  PubMed  CAS  Google Scholar 

  • Klug, A., Holmes, K.C., Finch, J.T. (1961). X-ray diffraction studies on ribosomes from various sources. J. Mol. Biol 3: 87–100.

    Article  PubMed  CAS  Google Scholar 

  • Kress, Y., Wittner, M., Rosenbaum, R.M. (1971). Sites of cytoplasmic ribonucleoprotein-filament assembly in relation to helical body formation in axenic trophozoites of Entamoeba histolytica. J. Cell Biol. 49: 773–784.

    Article  PubMed  Google Scholar 

  • Kühlbrandt, W., Unwin, P.N.T. (1982). Distribution of RNA and protein in crystalline eukaryotic ribosomes. J. Mol. Biol 156: 431–448.

    Article  PubMed  Google Scholar 

  • Lake, J.A., Slayter, H.S. (1972). Three-dimensional structure of the chromatoid body helix of Entamoeba invadens. J. Mol. Biol 66: 271–282.

    Article  PubMed  CAS  Google Scholar 

  • Langridge, R., Holmes, K.C. (1962). X-ray diffraction studies of concentrated gels of ribosomes from E. coli. J. Mol. Biol 5: 611–617.

    CAS  Google Scholar 

  • Leijonmarck, M., Eriksson, S.,Liljas, A. (1980). Crystal structure of a ribosomal component at 2.6 A resolution. Nature 286: 824–826.

    CAS  Google Scholar 

  • Leonard, K.R., Arad, T., Tesche, B., Erdmann, V.A., Wittmann, H.G., Yonath, A.E. (1981). Electron microscopy and three-dimensional reconstruction of 50S ribosomal subunits from the thin sections of crystals. 7th EMBO Symposium, Heidelberg, Abstract.

    Google Scholar 

  • Leonard, K.R., Arad, T., Tesche, B., Erdmann, V.A., Wittmann, H.G., Yonath, A.E. (1982). Crystallization, electron microscopy and three-dimensional reconstruction studies of ribosomal subunits. In: Electron Microscopy 1982. vol. 3. Offizin Paul Hartung, Hamburg, pp. 9–15.

    Google Scholar 

  • Leonard, K.R., Arad, T., Wittmann, H.G., Yonath, A.E. (1984). Structural studies on ribosomal particles. Eight Intern. Congress of Biophysics, Abstract.

    Google Scholar 

  • Liljas, A., Newcomer, M.E. (1981). Purification and crystallization of a protein complex from B. stearothermophilus. J. Mol. Biol, 153: 393–398.

    Article  PubMed  CAS  Google Scholar 

  • Marikowa, K. (1984). X-ray diffraction and electron microscopic study of 5S rRNA crystal. Eleventh Intern. Congress on Crystallography, Hamburg, Abstract.

    Google Scholar 

  • Milligan, R.A., Unwin, P.N.T. (1982). In vitro crystallization of ribosomes from chick embryos. J. Cell. Biol 95: 648–653.

    Article  PubMed  CAS  Google Scholar 

  • Miskin, R., Zamir, A., Elson, D. (1970). Inactivation and reactivation of ribosomal subunits: the peptidyl transferase activity of the 50S subunit of E. coli. J. Mol. Biol 54: 355–378.

    CAS  Google Scholar 

  • Moore, P.B., Kime, M.J., Leontis, N.B., Abdel-Meguid, S.S. (1983). Physical studies on a nucleoprotein from the ribosome of E. coli. J. Biomol. Struct. Dynam 1: 383–388.

    CAS  Google Scholar 

  • O’Brien, L., Shelley, K., Towfighi, J., McPherson, A. (1980). Crystalline ribosomes are present in brains of senile humans. Proc. Natl. Acad. Sci. USA 77: 2260–2264.

    Article  PubMed  Google Scholar 

  • Saper, M.A., Yonath, A., Wittmann, H.G. (1985). X-ray diffraction patterns from single crystals of the large ribosomal subunit from B. stearothermophilus. Abstr. Internat. Meeting on Ribosomes. Port Aransas, Texas.

    Google Scholar 

  • Shevack, A., Gewitz, H.S., Henneman, B., Yonath, A., Wittmann, H.G. (1985). Characterization and crystallization of ribosomal particles from Halobacteria marismortui. FEBS Lett. 184: 68–71.

    Article  CAS  Google Scholar 

  • Taddei, C. (1972). Ribosome arrangement during oogenesis of Lacerta sicula, Exp. Cell. Res 70: 285–292.

    Article  PubMed  CAS  Google Scholar 

  • Unwin, P.N.T. (1977). Three-dimensional model of membrane bound ribosomes obtained by electron microscopy. Nature 269: 118–122.

    Article  PubMed  CAS  Google Scholar 

  • Unwin, P.N.T., Taddei, C. (1977). Packing of ribosomes in crystals from the lizard Lacerta sicula. J. Mol. Biol 114: 491–506.

    Article  PubMed  CAS  Google Scholar 

  • White, S.W., Appelt, K., Dijk, J., Wilson, K.S. (1983). Proteins of the Bacillus stearothermophilus ribosome: a 5 Ã… structure analysis of protein S5. FEBS Lett. 163: 73–75.

    Article  PubMed  CAS  Google Scholar 

  • Wilson, K.S., Appelt, K., Badger, J., Tanaka, I., White, S.W. The crystal structure of an intact ribosomal protein. Submitted for publication. Wittmann, H.G. (1983). Architecture of prokaryotic ribosomes. Ann. Rev. Biochem 52: 35–65.

    Google Scholar 

  • Wittmann, H.G., Müssig, J., Gewitz, H.S., Piefke, J., Rheinberger, H.J., Yonath, A. (1982). Crystallization of E. coli ribosomes. FEBS Lett. 146: 217–220.

    Article  CAS  Google Scholar 

  • Wittmann, H.G., Yonath, A. (1985). Diffraction studies on crystals of ribosomal particles. In: The structure and function of the genetic apparatus, eds. Ts’o, Nicolini, Plenum Press, New York, pp. 177–189.

    Google Scholar 

  • Yonath, A., Müssig, J., Tesche, B., Lorenz, S., Erdmann, V.A., Wittmann, H.G. (1980). Crystallization of the large ribosomal subunit from B. stearothermophilus. Biochem. Internat. 1: 428–435.

    Google Scholar 

  • Yonath, A., Müssig, J., Wittmann, H.G. (1982a). Parameters for crystal growth of ribosomal subunits. J. Cell. Biochem 19: 629–639.

    Article  Google Scholar 

  • Yonath, A., Khavitch, G., Tesche, B., Müssig, J., Lorenz, S., Erdmann, V.A., Wittmann, H.G. (1982b). The nucleation of crystals of the large ribosomal subunits from B. stearothermophilus. Biochem. Internat 5: 629–636.

    CAS  Google Scholar 

  • Yonath, A., Tesche, B., Lorenz, S., Müssig, J., Erdmann, V.A., Wittmann, H.G. (1983 a). Several crystal forms of the 50 S ribosomal particles of B. stearothermophilus. FEBS Lett. 154: 15–20.

    Google Scholar 

  • Yonath, A., Piefke, J., Müssig, J., Gewitz, H.S., Wittmann, H.G. (1983b) A compact three-dimensional crystal form of the large ribosomal subunit from B. stearothermophilus. FEBS Lett. 163: 69–78.

    Article  PubMed  CAS  Google Scholar 

  • Yonath, A. (1984). Three-dimensional crystals of ribosomal particles. TIBS 9: 227–230.

    Google Scholar 

  • Yonath, A, Bartunik, H.D., Bartels, K.S., Wittmann, H.G. (1984). Some X-ray diffraction patterns from single crystals of the large ribosomal subunit from B. stearothermophilus. J. Mol. Biol 177: 201–206.

    Article  PubMed  CAS  Google Scholar 

  • Yonath, A., Saper, M.A., Makowski, I., Müssig, J., Piefke, J., Bartunik, H.D., Bartels, K.S., Wittmann, H.G. (1986). Characterization of single crystals of the large ribosomal particles from B. stearothermophilus. J. Mol. Biol 187: 633–636.

    Article  PubMed  CAS  Google Scholar 

  • Zubay, G., Wilkins, M.H.F. (1960). X-ray diffraction studies of the structure of ribosomes from E. coli. J. Mol. Biol 2: 105–112.

    CAS  Google Scholar 

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Authors
  1. A. Yonath
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  2. M. A. Saper
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  3. H. G. Wittmann
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Editor information

Editors and Affiliations

  1. Clayton Foundation Biochemical Institute Department of Chemistry, The University of Texas at Austin, 78712, Austin, Texas, USA

    Boyd Hardesty  & Gisela Kramer  & 

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Yonath, A., Saper, M.A., Wittmann, H.G. (1986). Studies on Crystals of Intact Bacterial Ribosomal Particles. In: Hardesty, B., Kramer, G. (eds) Structure, Function, and Genetics of Ribosomes. Springer Series in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4884-2_7

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  • DOI: https://doi.org/10.1007/978-1-4612-4884-2_7

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9346-0

  • Online ISBN: 978-1-4612-4884-2

  • eBook Packages: Springer Book Archive

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