Abstract
Ribosomal RNA must have a strategic and important role that cannot be played by ribosomal proteins, nor perhaps by any other cellular component. The structural complexity of the ribosomal RNAs is not merely a gratuitous gesture of nature but is indicative of strong structural and functional roles. For these reasons, it is essential that the structural and functional domains of the ribosomal RNA within the ribosome be clearly defined and mapped.
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References
Backendorf, C., Overbeek, G.P., Van Boom, J.H., Van der Marel, G., Veeneman, G., Van Duin, J. (1980). Role of 16S RNA in ribosome messenger recognition. Eur. J. Biochem. 110: 599–604.
Barta, A., Steiner, G., Brosius, J., Noller, H.F., Kuechler, E. (1984). Identification of a site on 23S ribosomal RNA located at the peptidyl transferase center. Proc. Natl. Acad. Sci. USA 81: 3607–3611.
Berkower, I., Leis, J., Hurwitz, J. (1973). Isolation and characterization of an endonuclease from E. coli specific for ribonucleic acid-deoxyribonucleic acid hybrid structures. J. Biol. Chem. 248: 5914–5921.
Brow, D.A., Noller, H.F. (1983). Protection of ribosomal RNA from kethoxal in polyribosomes. Implication of specific sites in ribosome function. J. Mol. Biol. 163: 27–46.
Donis-Keller, H. (1979). Site specific enzymatic cleavage of RNA. Nucl. Acids Res. 7: 179–192.
Douthwaite, S., Christensen, A., Garrett, R.A. (1983). Higher order structure in the 3′-minor domain of small subunit ribosomal RNAs from a gram negative bacterium, a gram positive bacterium and a eukaryote. J. Mol. Biol. 169: 249–279.
Herr, W., Noller, H.F. (1979) Protection of specific sites in 23S and 5S RNA from chemical modification by association of 30S and 50S ribosomes. J. Mol. Biol. 130: 421–432.
Mankin, A.S., Skripkin, E.A., Chichkova, N.V., Kopylov, A.M., Bogdanov, A.A. (1981). An enzymatic approach for localization of oligodeoxyribonucleotide binding sites on RNA. FEBS Lett. 131: 253–256.
Meier, N., Wagner, R. (1984). Binding of tRNA alters the chemical accessibility of nucleotides within the large ribosomal RNAs of E. coli ribosomes. Nucl. Acids Res. 12: 1473–1487.
Noller, H.F. (1974). Topography of 16S RNA in 30S ribosomal subunits. Nucleotide sequences and location of sites of reaction with kethoxal. Biochemistry 13: 4694–4703.
Noller, H.F. (1984). Structure of ribosomal RNA. Ann. Rev. Biochem. 53: 119-162.
Noller, H.F., Chaires, J.B. (1972). Functional modification of 16S ribosomal RNA by kethoxal. Proc. Natl. Acad. Sci. USA 69: 3115–3118.
Noller, H.F., Kop, J., Wheaton, V., Brosius, J., Guteil, R.R., Kopylov, A.M., Dohme, F., Herr, W. (1981). Secondary structure model for 23S ribosomal RNA. Nucl. Acids Res. 9: 6167–6189.
Ofengand, J., Liou, R., Kohut, J., Ill, Schwartz, I., Zimmermann, R.A. (1979). Covalent cross-linking of transfer ribonucleic acid to the ribosomal P site. Mechanism and site of reaction in transfer ribonucleic acid. Biochemistry 18: 4322–4332.
Peattie, D., Gilbert, W. (1980). Chemical probes for higher-order structure in RNA. Proc. Natl. Acad. Sci. USA 77: 4679–4682.
Peattie, D., Herr, W. (1981). Chemical probing of the tRNA-ribosome complex. Proc. Natl. Acad. Sci. USA 78: 2273–2277.
Prince, J.B., Taylor, B.H., Thurlow, D.L., Ofengand, J., Zimmermann, R.A. (1982). Covalent crosslinking of tRNAval1 to 16S RNA at the ribosomal P site: identification of the crosslinked residues. Proc. Natl. Acad. Sci. USA 79: 5450–5454.
Santer, M., Shane, S. (1977). Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of E. coli. J. Bact. 130: 900–910.
Schwartz, I., Ofengand, J. (1978). Photochemical cross-linking of unmodified acetyl- valyl-tRNA to 16S RNA at the ribosomal P site. Biochemistry 17: 2524–2530.
Skripkin, E.A., Kagramanova, V.K., Chichkova, N.V., Loplylov, A.M., Bogdanov, A.A. (1981). Mapping of the 16S rRNA regions in the ribosome capable of complementary binding of oligonucleotides. Biokhimiya 46: 2250–2256.
Taylor, B.N., Prince, J.B., Ofengand, J., Zimmermann, R.A. (1981). Nonanucleotide sequence from 16S ribonucleic acid at the peptidyl transfer ribonucleic acid binding site of the Escherichia coli ribosome. Biochemistry 20: 7581–7588.
Van Stolk, B.J., Noller, H.F. (1984) Chemical probing of conformation in large RNA molecules: analysis of 16S ribosomal RNA using diethylpyrocarbonate. J. Mol. Biol. 180: 151–177.
Vassilenko, S.K., Carbon, P., Ebel, J.P., Ehresmann, C. (1981). Topography of 16S RNA in 30S subunits and 70S ribosomes accessibility to cobra venom ribonuclease. J. Mol.Biol. 152: 699–721.
Woese, C.R., Gutell, R, Gupta, R, Noller, H.F. (1983). Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol. Rev. 47: 621–669.
Zimmermann, R.A., Gates, S.M., Schwartz, I., Ofengand, J. (1979). Covalent cross-linking of transfer ribonucleic acid to the ribosomal P site. Site of reaction in 16S ribonucleic acid. Biochemistry 18: 4333–4339.
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Hill, W.E., Tapprich, W.E., Tassanakajohn, A. (1986). Probing Ribosomal Structure and Function. In: Hardesty, B., Kramer, G. (eds) Structure, Function, and Genetics of Ribosomes. Springer Series in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4884-2_14
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DOI: https://doi.org/10.1007/978-1-4612-4884-2_14
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