Abstract
The one-dimensional isoelectric focusing (1D-IEF) patterns for HLA-A and HLA-B antigens for the cells in the Reference Panel of B-lymphoblastoid cell lines provide a permanent record for individual cell lines that can be used as reference cells in future studies. Table 1 shows the phenotyping of the cell line panel assigned by 1D-IEF typing. Designations of the IEF assignments are explained in detail in the Workshop summary report in the HLA Class I Biochemistry component in this volume. Briefly, decimal numbers were used to designate subtypes or variants for each antigen, starting from the top of the gel (cathode) to the bottom (anode) of the gel, keeping the original serologic specificities. For example, B17.2, Bw57.2, and Bw58.2 have the same IEF band and designate the second band from the top of the gel among the bands that belong to the B17 antigen group.
Participating Laboratory: US7SYY1
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© 1989 Springer-Verlag New York
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Yang, S.Y. (1989). Assignment of HLA-A and HLA-B Antigens for the Reference Panel of B-Lymphoblastoid Cell Lines Determined by One-Dimensional Isoelectric Focusing (ID-IEF) Gel Electrophoresis. In: Dupont, B. (eds) Immunobiology of HLA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3552-1_5
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DOI: https://doi.org/10.1007/978-1-4612-3552-1_5
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