Abstract
This method provides a means with which to study the development of intact maize kernels under conditions in which nutritional, hormonal, environmental and other factors can be modified and controlled. Kernels may be cultured from 2 days after pollination up to physiological maturity. Final kernel size may vary, but average kernel dry weight generally ranges from 70% to 100% of normal.
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References
Culley DE, Gengenbach BG, Smith JA, Rubenstein I, Connelly JA, Park WD (1984) Endosperm protein synthesis and L-[35-S]methionine incorporation in maize kernels cultured In vitro. Plant Physiol 74: 389–394
Gengenbach BG (1977) Development of maize caryopses resulting from in-vitro pollination. Planta 134: 91–93
Gengenbach BG (1984) In vitro pollination, fertilization and development of maize kernels. In Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol. 1, Academic Press, New York, pp 276–282
Singletary GW, Below FE (1989) Growth and composition of maize kernels cultured in vitro with varying supplies of carbon and nitrogen. Plant Physiol 89: 341–346
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© 1994 Springer-Verlag New York, Inc.
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Gengenbach, B.G., Jones, R.J. (1994). In vitro Culture of Maize Kernels. In: Freeling, M., Walbot, V. (eds) The Maize Handbook. Springer Lab Manuals. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2694-9_127
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DOI: https://doi.org/10.1007/978-1-4612-2694-9_127
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-94735-8
Online ISBN: 978-1-4612-2694-9
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