Summary
Thermal injuries were induced on the back of young pigs. After two to three days each wound was inoculated with 109 to 5×109 Staphylococcus aureus cells. The wounds were covered by an occlusive wound dressing for 3 days and then treated for 5 days with daily changes of dressing. In wounds treated with fibronectin-substituted Sepharose 6B or DEAE-Sephadex there was a significant decrease in the bacterial count on the third day compared to wounds treated with unsubstituted gels. On the fourth day of treatment these wounds showed young granulation tissue throughout dermis whereas wounds treated with unsubstituted gels still presented as an acute inflammation. Macroscopically, wounds treated with fibronectin-substituted gels healed more rapidly than control wounds.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alvarez OM, Mertz PM, Eaglstein WH.: Benzoyl peroxide and epidermal wound healing. Arch Dermatol 119: 222–225, 1983.
Johnsson P, Wadström T.: Cell surface hydrophobicity of Staphylococcus aureus measured by the salt aggregation test (SAT). Curr Microbiol 10: 203–210; 1984.
Ljungh A, Hjertén S, Wadström T.: High surface hydrophobicity of autoaggregating Staphylococcus aureus strains isolated from human infections. Infect Immun 47: 522–526; 1985.
Loebl EC, Marvin JA, Heck EL, Curreri PW, Baxter CR.: The method of quantitative burn-wound biopsy cultures and its routine use in the care of the burned patient. Am J Qin Pathol 61: 20–24; 1974.
Maze I, Rydén C, Wadström T, Rubin K.: Specific attachment of Staphylococcus aureus to immobilized fibronectin. Infect Immun 54: 695–704; 1986.
Mertz PM, Hebda PA, Eaglstein WH.: A porcine model for evaluating epidermal wound healing, in Tumbleson ME (ed) Swine in Biomedical research, Plenum Publ Co, New York, vol.1, pp. 291–302, 1986.
Mertz PM, Patti JM, Marcin JJ, Marshall DA.: Model for studying bacterial adherence to skin wounds. J Clin Microbiol 25: 1601–1604; 1987.
Mosher DF: Fibronectin. Academic Press Inc, San Diego, New York, Berkeley, Boston, 1989.
Nyström P: Quantitation of bacteria in operating wounds-an experimental investigation with swab sampling in pigs. Acta path microbiol scand B 88: 71–77; 1980.
Sandén G, Ljungh A, Wadström T, Banck G, Miedzubrodski J, Svedman P.: Staphylococcal wound infection in the pig II. Inoculation, quantification of bacteria and reproducibility. Ann Plastic Surgery 23: 219–230: 1989.
Wadström T, Björnberg S, Hjertén S.: Hydrophobized wound dressing in the treatment of experimental Staphylococcus aureus infections in the young pig. Acta path microbiol scand B 93: 359–363; 1985.
Wadström T, Switalski LM, Speziale P, Rubin K, Rydén C, Fröman G, Faris A, Lindberg M, Höök M.: Binding of microbial pathogens to connective tissue fibronectin: An early step in localized and invasive infections, in Jackson GG (ed) The pathogenesis of bacterial infections, Springer Verlag, Berlin,pp. 193–200, 1985.
Williamson P, Kligman AM.: A new method for the quanti taive investigation of cutaneous bacteria. J Investig Derma tol 45: 498–503; 1965.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag London Limited
About this chapter
Cite this chapter
Ljungh, Å., Kronevi, T., Wadström, T. (1990). Fibronectin-Substituted Gels for Treatment of Experimental Wound Infections in a Pig Model. In: Wadström, T., Eliasson, I., Holder, I., Ljungh, Å. (eds) Pathogenesis of Wound and Biomaterial-Associated Infections. Springer, London. https://doi.org/10.1007/978-1-4471-3454-1_20
Download citation
DOI: https://doi.org/10.1007/978-1-4471-3454-1_20
Publisher Name: Springer, London
Print ISBN: 978-3-540-19596-2
Online ISBN: 978-1-4471-3454-1
eBook Packages: Springer Book Archive