Abstract
PCR has been the most important invention of the past decade which has revolutionized the field of molecular biology. Beginning with a single molecular of DNA, the PCR can generate billions of copies of DNA in few hours, i.e. Nano gram(ng) of DNA can be amplified to get μg of DNA by using this technique. PCR technique is based on in vitro enzymatic amplification of a specific target DNA sequence in a cyclic process using two oligonucleotides. These oligos used as primers have different sequences and are complementary to the sequences on the opposite strands of the template DNA and flank the segment of target DNA that is to be amplified. Thus, given a particular target DNA, large amounts of that product and only that product are produced in sufficient quantities for subsequent experimental analysis.
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Katoch, R. (2011). Techniques in Molecular Biology. In: Analytical Techniques in Biochemistry and Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9785-2_15
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DOI: https://doi.org/10.1007/978-1-4419-9785-2_15
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