Abstract
The optical microscope is used extensively in pharmaceutical development with the primary application being solid-state analysis. The applications range from simple images of drug substance to illustrate particle size and shape to full optical crystallography. The range of utility of the microscope is considerably extended by the use of polarized light which allows us to obtain crystallographic data on small individual crystals. I will use the term Polarized Light Microscopy (PLM) for all light microscopy discussions in this chapter.
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References
Aldrich DS, Smith MA (1995) Pharmaceutical Applications of Infrared Microspectroscopy. In: Humeki HJ (ed) Practical Guide to Infrared Microspectroscopy. Marcel Dekkar, New York
Allen RM (1962) Practical Refractometry By Means of the Microscope, 2nd Edition. Cargille Laboratories, Cedar Grove NJ USA
Bloss FD (1961) An Introduction to the Methods Of Optical Crystallography. Holt, Rinehart and Winston, New York
Bloss FD (1971) Crystallography and Crystal Chemistry: An Introduction. Holt, Rinehart and Winston, New York
Bloss FD (1981) The Spindle Stage: Principles and Practice. Cambridge University Press, Cambridge
Born M, Wolf E (1980) Principles of Optics, 6th Edition. Pergamon Press, Oxford
Bowen AM (2009) Optical Crystallography of Silver Sulfadiazine. The Microscope 57, 1:11–18
Chamot EM, Mason CW (1958) Handbook of Chemical Microscopy, Vol. 1. John Wiley & Sons, New York
Delly JG (1988) Photography Through the Microscope. 9th edition. Kodak Publication P-2. Eastman Kodak Company, Rochester NY
Edwards HGM, Lawson E, de Matas M, Shields L, York P (1997) Metamorphosis of Caffeine Hydrate and Anhydrous Caffeine. J Chem Soc, Perkin Trans 2:1985-90
Feynman RP, Leighton RB, Sands M (1965) The Feynman Lectures on Physics, Vol. III, Chapters 1 – 3. Addison-Wesley, Reading Mass. USA
Gage SH (1943) The Microscope, 17th Edition. Comstock Publishing, Ithaca
Gunter ME, Bandli BR, Bloss FD, Evans SH, Su SC, Weaver R (2004) Results From a McCrone Spindle Stage Short Course, a New Version of EXCALIBR, and How to Build a Spindle Stage. The Microscope 52, 1:23-39
Hartshorne NH, Stuart A (1970) Crystals and the Polarizing Microscope, 4th Edition. American Elsevier, New York
Inoué S (1986) Video Microscopy. Plenum Press, New York
Martin LC (1966) The Theory of the Microscope. American Elsevier, New York
Mason CW (1945) Recommended Practice for Microscopical Reports on Crystalline Materials in ACS Publications. Industrial and Engineering Chemistry, Vol 17, No 9:603–  4
McCrone WC, McCrone LB, Delly JG (1984) Polarized Light Microscopy. McCrone Research Institute, Chicago
McKie D, McKie C (1986) Essentials of Crystallography. Blackwell, London
Merriam Webster (1983) Websters Ninth New Collegiate Dictionary, Merriam Webster
Matsumoto B (2010) Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences. Rocky Nook, Inc. Santa Barbara, CA, USA
Murphy DB (2001) Fundamentals of Light Microscopy and Electronic Imaging. Wiley, Hoboken NJ
Needham GH (1958) The Practical Use of The Microscope. Charles C Thomas, Springfield
Nichols G (1998) Optical Properties of Polymorphic Forms I and II of Paracetamol. The Microscope 46, 3:117-122.
Nichols G (2006) Light Microscopy. In: Hilfiker R (ed) Polymorphism in the Pharmaceutical Industry. Wiley, New York
Nichols G, Frampton C (1998) Physicochemical Characterization of the Orthorhombic Polymorph of Paracetamol Crystallized from Solution. J Pharm Sci 87, 6: 684–693
Slayter EM, Slayter HS (1992) Light and Electron Microscopy. Cambridge University Press
Stoiber RE, Morse SA (1994) Crystal Identification with the Polarizing Microscope. Chapman and Hall, New York
Wahlstrom EE (1979) Optical Crystallography, 5th edition. John Wiley & Sons, New York
Weaver JH (1987) Albert Einstein: Development of Our Conception of the Nature and Constitution of Radiation. In The World of Physics: A Small Library of the Literature of Physics from Antiquity to the Present. Volume I. Simon and Shuster, New York
Wood EA (1977) Crystals and Light, 2nd Revised Edition. Dover, New York
Internet References
Delly JG (2003a) Sénarmont Compensation: How to Accurately Measure Small Relative Retardations (0-1λ). http://www.modernmicroscopy.com/main.asp?article=14&page=1
Delly JG (2003b) The Michel-Lévy Interference Color Chart – Microscopy’s Magical Color Key. http://www.modernmicroscopy.com/main.asp?article=15
Microscopy – UK. http://www.microscopy-uk.org.uk/index.html
Microtrace, LLC. http://www.microtracescientific.com/index.html
Molecular Expressions: Optical Microscopy Primer. http://micro.magnet.fsu.edu/primer/anatomy/anatomy.html
Nikon Microscopy Education. http://www.microscopyu.com/
Olympus Microscopy Resource Center. http://www.olympusmicro.com/index.html
Carl Zeiss, Inc. http://www.zeiss.com/
Zeiss Brochure: Michel Levy Color Chart, Polarized Light, Conoscopic Determination. http://www.zeiss.com/C1256D18002CC306/0/F2BA0A81B5929487C1256D59003351AA/$file/46-0014_e.pdf
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Carlton, R.A. (2011). Polarized Light Microscopy. In: Pharmaceutical Microscopy. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-8831-7_2
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DOI: https://doi.org/10.1007/978-1-4419-8831-7_2
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