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Sialylated and Sulfated Carbohydrate Ligands for Selectins and Siglecs: Involvement in Traffic and Homing of Human Memory T and B Lymphocytes

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The Molecular Immunology of Complex Carbohydrates-3

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 705))

Abstract

Selectin-mediated adhesion to endothelial cells is the first step of extravasation of leukocytes. Neutrophils constitutively express sialyl Lewis x, the selectin ligand, and are ready to adhere to endothelial cells once the latter cells express E- or P-selectin. This leads to rapid and massive extravasation of neutrophils into acute inflammatory lesions.

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Abbreviations

BCR:

B-cell antigen receptor

CCR:

CC chemokine receptor

CD:

Cluster of differentiation

CLA:

Cutaneous lymphocyte antigen

ELISA:

Enzyme-linked immunosorbent assay

Fuc-T:

Fucoyltransferase

HDAC:

Histone deacetylase

ITIM:

Immunoreceptor tyrosine-based inhibition motif

NF:

Nuclear factor

NK:

Natural killer

siglec:

Sialic acid binding immunoglobulin (Ig)-like lectins

SLC:

Secondary lymphoid-tissue chemokine

TARC:

Thymus and activation-regulated chemokine

Th1:

T-helper 1

Th2:

T-helper 2

TNF:

Tumor necrosis factor

TPA:

12-O-Tetradecanoylphorbol-13-acetate

References

  1. Stroud MR, Handa K, Salyan ME, Ito K, Levery SB, Hakomori S, Reinhold BB, Reinhold WN (1996) Monosialogangliosides of human myelogenous leukemia HL60 cells and normal human leukocytes. 1. Separation of E-selectin binding from nonbinding gangliosides, and absence of sialosyl-Lex having tetraosyl to octaosyl core. Biochemistry 35:758–769

    Article  PubMed  CAS  Google Scholar 

  2. Stroud MR, Handa K, Salyan ME, Ito K, Levery SB, Hakomori S, Reinhold BB, Reinhold VN (1996) Monosialogangliosides of human myelogenous leukemia HL60 cells and normal human leukocytes. 2. Characterization of E-selectin binding fractions, and structural requirements for physiological binding to E-selectin. Biochemistry 35:770–778

    Article  PubMed  CAS  Google Scholar 

  3. Withers DA, Hakomori SI (2000) Human α(1, 3)-fucosyltransferase IV (FUTIV) gene expression is regulated by elk-1 in the U937 cell line. J Biol Chem 275:40588–40593

    Article  PubMed  CAS  Google Scholar 

  4. Nimrichter L, Burdick MM, Aoki K, Laroy W, Fierro MA, Hudson SA, Von Seggern CE, Cotter RJ, Bochner BS, Tiemeyer M, Konstantopoulos K, Schnaar RL (2008) E-selectin receptors on human leukocytes. Blood 112:3744–3752

    Article  PubMed  CAS  Google Scholar 

  5. Kannagi R (2001) Transcriptional regulation of expression of carbohydrate ligands for cell adhesion molecules in the selectin family. In: Wu AM (ed) Advances in experimental medicine and biology, vol 491, Molecular immunology of complex carbohydrates II. Plenum Press, New York, pp 267–278

    Google Scholar 

  6. Kannagi R (2002) Regulatory roles of carbohydrate ligands for selectins in homing of lymphocytes. Curr Opin Struct Biol 12:599–608

    Article  PubMed  CAS  Google Scholar 

  7. Hiraiwa N, Yabuta T, Yoritomi K, Hiraiwa M, Tanaka Y, Suzuki T, Yoshida M, Kannagi R (2003) Trans-activation of the fucosyltransferase VII gene by human T-cell leukemia virus type 1 tax through a variant cAMP-responsive element. Blood 101:3615–3621

    Article  PubMed  CAS  Google Scholar 

  8. Koike T, Kimura N, Miyazaki K, Yabuta T, Kumamoto K, Takenoshita S, Chen J, Kobayashi M, Hosokawa M, Taniguchi A, Kojima T, Ishida N, Kawakita M, Yamamoto H, Takematsu H, Kozutsumi Y, Suzuki A, Kannagi R (2004) Hypoxia induces adhesion molecules on cancer cells – a missing link between Warburg effect and induction of selectin ligand carbohydrates. Proc Natl Acad Sci USA 101:8132–8137

    Article  PubMed  CAS  Google Scholar 

  9. Chen GY, Osada H, Santamaria-Babi LF, Kannagi R (2006) Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes. Proc Natl Acad Sci USA 103:16894–16899

    Article  PubMed  CAS  Google Scholar 

  10. Austrup F, Vestweber D, Borges E, Löhning M, Bräuer R, Herz U, Renz H, Hallmann R, Scheffold A, Radbruch A, Hamann A (1997) P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflamed tissues. Nature 385:81–83

    Article  PubMed  CAS  Google Scholar 

  11. Mitsuoka C, Sawada-Kasugai M, Ando-Furui K, Izawa M, Nakanishi H, Nakamura S, Ishida H, Kiso M, Kannagi R (1998) Identification of a major carbohydrate capping group of the L-selectin ligand on high endothelial venules in human lymph nodes as 6-sulfo sialyl Lewis x. J Biol Chem 273:11225–11233

    Article  PubMed  CAS  Google Scholar 

  12. Kimura N, Mitsuoka C, Kanamori A, Hiraiwa N, Uchimura K, Muramatsu T, Tamatani T, Kansas GS, Kannagi R (1999) Reconstitution of functional L-selectin ligands on a cultured human endothelial cell line by co-transfection of α1→3 fucosyltransferase VII and newly cloned GlcNAcβ: 6-sulfotransferase Cdna. Proc Natl Acad Sci USA 96:4530–4535

    Article  PubMed  CAS  Google Scholar 

  13. Ohmori K, Kanda K, Mitsuoka C, Kanamori A, Kurata-Miura K, Sasaki K, Nishi T, Tamatani T, Kannagi R (2000) P- and E-selectins recognize sialyl 6-sulfo Lewis X, the recently-identified L-selectin ligand. Biochem Biophys Res Commun 278:90–96

    Article  PubMed  CAS  Google Scholar 

  14. Kanamori A, Kojima N, Uchimura K, Muramatsu T, Tamatani T, Berndt MC, Kansas GS, Kannagi R (2002) Distinct sulfation requirements of selectins disclosed using cells that support rolling mediated by all three selectins under shear flow. J Biol Chem 277:32578–32586

    Article  PubMed  CAS  Google Scholar 

  15. Ohmori K, Fukui F, Kiso M, Imai T, Yoshie O, Hasegawa H, Matsushima K, Kannagi R (2006) Identification of cutaneous lymphocyte-associated antigen as sialyl 6-sulfo Lewis x, a selectin ligand expressed on a subset of skin-homing helper memory T cells. Blood 107:3197–3204

    Article  PubMed  CAS  Google Scholar 

  16. Mitsuoka C, Ohmori K, Kimura N, Kanamori A, Komba S, Ishida H, Kiso M, Kannagi R (1999) Regulation of selectin binding activity by cyclization of sialic acid moiety of carbohydrate ligands on human leukocytes. Proc Natl Acad Sci USA 96:1597–1602

    Article  PubMed  CAS  Google Scholar 

  17. Berg EL, Yoshino T, Rott LS, Robinson MK, Warnock RA, Kishimoto TK, Picker LJ, Butcher EC (1991) The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med 174:1461–1466

    Article  PubMed  CAS  Google Scholar 

  18. Picker LJ, Michie SA, Rott LS, Butcher EC (1990) A unique phenotype of skin-associated lymphocytes in humans. Preferential expression of the HECA-452 epitope by benign and malignant T cells at cutaneous sites. Am J Pathol 136:1053–1068

    PubMed  CAS  Google Scholar 

  19. Ohmori K, Takada A, Ohwaki I, Takahashi N, Furukawa Y, Maeda M, Kiso M, Hasegawa A, Kannagi M, Kannagi R (1993) A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively expressed on helper memory T cells. Blood 82:2797–2805

    PubMed  CAS  Google Scholar 

  20. Lanzavecchia A, Sallusto F (2005) Understanding the generation and function of memory T cell subsets. Curr Opin Immunol 17:326–332

    Article  PubMed  CAS  Google Scholar 

  21. Sallusto F, Geginat J, Lanzavecchia A (2004) Central memory and effector memory T cell subsets: function, generation, and maintenance. Annu Rev Immunol 22:745–763

    Article  PubMed  CAS  Google Scholar 

  22. Razi N, Varki A (1998) Masking and unmasking of the sialic acid-binding lectin activity of CD22 (Siglec-2) on B lymphocytes. Proc Natl Acad Sci USA 95:7469–7474

    Article  PubMed  CAS  Google Scholar 

  23. Chen GY, Sakuma K, Kannagi R. Manuscript in preparation.

    Google Scholar 

  24. Tjew SL, Brown KL, Kannagi R, Johnson P (2005) Expression of N-acetylglucosamine 6-O sulfotransferases (GlcNAc6STs) -1 and -4 in human monocytes: GlcNAc6ST-1 is implicated in the generation of the 6-sulfo N-acetyllactosamine/Lewis x epitope on CD44 and is induced by TNF-α. Glycobiology 15:7C–13C

    Article  PubMed  CAS  Google Scholar 

  25. Chen GY, Sakuma K, Kannagi R (2008) Significance of NF-κB/GATA axis in TNF-α-induced expression of 6-sulfated cell-recognition glycans in human T-lymphocytes. J Biol Chem 283:34563–34570

    Article  PubMed  CAS  Google Scholar 

  26. Das J, Chen CH, Yang L, Cohn L, Ray P, Ray A (2001) A critical role for NF-κB in GATA3 expression and TH2 differentiation in allergic airway inflammation. Nat Immunol 2:45–50

    Article  PubMed  CAS  Google Scholar 

  27. Boothby M (2001) Specificity of sn50 for NF-κB? Nat Immunol 2:471–472

    Article  PubMed  CAS  Google Scholar 

  28. Farrar JD, Asnagli H, Murphy KM (2002) T helper subset development: roles of instruction, selection, and transcription. J Clin Invest 109:431–435

    PubMed  CAS  Google Scholar 

  29. Blixt O, Head S, Mondala T, Scanlan C, Huflejt ME, Alvarez R, Bryan MC, Fazio F, Calarese D, Stevens J, Razi N, Stevens DJ, Skehel JJ, van Die I, Burton DR, Wilson IA, Cummings R, Bovin N, Wong CH, Paulson JC (2004) Printed covalent glycan array for ligand profiling of diverse glycan binding proteins. Proc Natl Acad Sci USA 101:17033–17038

    Article  PubMed  CAS  Google Scholar 

  30. Kimura N, Ohmori K, Miyazaki K, Izawa M, Matsuzaki Y, Yasuda Y, Takematsu H, Kozutsumi Y, Moriyama A, Kannagi R (2007) Human B-lymphocytes express α 2-6 sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/siglec-2. J Biol Chem 282:32200–32207

    Article  PubMed  CAS  Google Scholar 

  31. Wakabayashi C, Adachi T, Wienands J, Tsubata T (2002) A distinct signaling pathway used by the IgG-containing B cell antigen receptor. Science 298:2392–2395

    Article  PubMed  CAS  Google Scholar 

  32. Horikawa K, Martin SW, Pogue SL, Silver K, Peng K, Takatsu K, Goodnow CC (2007) Enhancement and suppression of signaling by the conserved tail of IgG memory-type B cell antigen receptors. J Exp Med 204:759–769

    Article  PubMed  CAS  Google Scholar 

  33. Waisman A, Kraus M, Seagal J, Ghosh S, Melamed D, Song J, Sasaki Y, Classen S, Lutz C, Brombacher F, Nitschke L, Rajewsky K (2007) IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig α/β. J Exp Med 204:747–758

    Article  PubMed  CAS  Google Scholar 

  34. Hanasaki K, Varki A, Powell LD (1995) CD22-mediated cell adhesion to cytokine-activated human endothelial cells. Positive and negative regulation by α-2-6-sialylation of cellular glycoproteins. J Biol Chem 270:7533–7542

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported in part by a grant-in-aid from the Ministry of Education, Science, Sports and Culture in Japan (21590324); grants-in-aid for the Third Term Comprehensive Ten-Year Strategy for Cancer Control from the Ministry of Health and Welfare, Japan; and grants from the Uehara Memorial Foundation, and the Nagano Medical Foundation, Japan.

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Authors and Affiliations

  1. Research Complex for the Medical Frontiers, Aichi Medical University, Aichi, 480-1195, Japan

    Reiji Kannagi

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  1. Reiji Kannagi
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  2. Katsuyuki Ohmori
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  3. Guo-Yun Chen
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  4. Keiko Miyazaki
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  5. Mineko Izawa
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  6. Keiichiro Sakuma
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Correspondence to Reiji Kannagi .

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  1. College of Medicine, Institute of Molecular&Cellular Biology, Chang-Gung University, Kwei-San, Tao-Yuan, 333, Taiwan R.O.C.

    Albert M. Wu

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Kannagi, R., Ohmori, K., Chen, GY., Miyazaki, K., Izawa, M., Sakuma, K. (2011). Sialylated and Sulfated Carbohydrate Ligands for Selectins and Siglecs: Involvement in Traffic and Homing of Human Memory T and B Lymphocytes. In: Wu, A. (eds) The Molecular Immunology of Complex Carbohydrates-3. Advances in Experimental Medicine and Biology, vol 705. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7877-6_29

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