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Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro

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Biomphalaria Snails and Larval Trematodes

Abstract

This chapter describes the methods used by the current authors and numerous other investigators to optimize conditions for successfully growing and maintaining both uninfected and infected Biomphalaria glabrata in the laboratory, with particular emphasis on the optimal physical, chemical, and biological factors that affect successful snail rearing and/or breeding, and the ways that they can be measured. Consideration is also given to incidental organisms that can alter aquarium ecology and affect both the snails and their intended trematode parasites. Various methods of infecting snails and maintaining snail tissues and cells in vitro are also described. The goal of this chapter is to aid researchers who have the need or desire to maintain the life cycle of this pulmonate and its trematode parasites in the laboratory.

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Authors and Affiliations

  1. Department of Biological Sciences, California State University, Long Beach, CA, 90840, USA

    L. K. Eveland

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  1. L. K. Eveland
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  2. M. A. Haseeb
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Correspondence to L. K. Eveland .

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  1. , Departamento de Biología Celular y Paras, Universidad de València, Av. Vicent Andrés Estellés s/n s/n, Burjassot, 46100, Valencia, Spain

    Rafael Toledo

  2. , Department of Biology, Lafayette College, Easton, 18042, Pennsylvania, USA

    Bernard Fried

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Eveland, L.K., Haseeb, M.A. (2011). Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro. In: Toledo, R., Fried, B. (eds) Biomphalaria Snails and Larval Trematodes. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7028-2_2

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