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Low-temperature preservation of fish gonad cells and oocytes

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The Fish Oocyte

Ensuring the retention of a record of the genetic diversity of species involves, at its simplest form, the long-term storage of material from somatic and reproductive cell lines. However, to have real practical benefit such material should be in the form of viable cellular material, ideally from the germline or reproductive cell lines. The ability to generate banks of cryopreserved sperm, eggs, and embryos that retain full viability following recovery from the frozen state would be the most powerful facilitating tool in species conservation and commercial applications such as aquaculture. The nuclear genomes of fish show a full range of sex chromosome differentiation, ranging from the all-autosomal karyotype as seen in the zebrafish to the genetically and cytogenetically differentiated sex chromosomes seen in the guppy (Traut and Winking, 2001). The preservation of haploid genomes in the viable form of male and female reproductive cells, or of viable embryos, is vital if normal populations of males and females are to be established.

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Authors and Affiliations

  1. Luton Institute of Research in the Applied Natural Sciences, University of Luton, 2 Adelaide Street, LU1 5DU, Luton, Bedfordshire, UK

    Tiantian Zhang & David M. Rawson

  2. National Institute of Oceanography, Israel Oceanographic and Limnological Research, 8020, 31080, Haifa, Israel

    Irena Pekarsky, Idit Blais & Esther Lubzens

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  1. Université Bordeaux 1, UMR NuAGe, 33405, Talence, France

    Patrick J. Babin

  2. IRTA, Institute of Marine Sciences of Barcelona, CSIC, and Center of Aquaculture, 08003, Barcelona, Spain

    Joan Cerdà

  3. National Institute of Oceanography, Israel Oceanographic and Limnological Research, 8020, 31080, Haifa, Israel

    Esther Lubzens

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Zhang, T., Rawson, D.M., Pekarsky, I., Blais, I., Lubzens, E. (2007). Low-temperature preservation of fish gonad cells and oocytes. In: Babin, P.J., Cerdà, J., Lubzens, E. (eds) The Fish Oocyte. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6235-3_14

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