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BIO-AEROSOL FLUORESCENCE

Detecting and characterising bio-aerosols via UV light-induced fluorescence spectroscopy

  • Conference paper
Optics of Biological Particles

Part of the book series: NATO Science Series ((NAII,volume 238))

Abstract

All aspects of fluorescence from a single or a cluster of bio-aerosol particles are treated in this chapter. The following topics are covered: light sources to induce fluorescence, detectors to sense fluorescence, excitation and emission spectra from simulants of known threat bio-aerosols, standards for monitoring aerosols, performance metrics, generation of dry and wet simulant bioaerosols, examples of commercially available bio-sensors, techniques for enriching aerosol samples by aerodynamic puffing, and the need for an identifier following the enrichment of aerosols sorted according to the aerosols’ fluorescence spectra. There is general agreement that fluorescence is a discriminator capable of separating bio- from non-bio-aerosols; however, an appropriate particle identifier has yet to be fully adapted to the monitoring of threat aerosols in the highly variable atmospheric environment.

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Authors and Affiliations

  1. Department of Applied Physics and Center for Laser Diagnostics, Yale University New Haven, 06520, Connecticut, USA

    Yong-Le Pan & Richard K. Chang

  2. US Naval Research Laboratory, Code 5611, 4555 Overlook Ave SW, 20375, Washington, DC, USA

    Jay D. Eversole, Alan Huston & Vasanthi Sivaprakasam

  3. Science & Technology Research Institute, University of Hertfordshire Hatfield, Herts. AL10 9AB, U.K.

    Paul H. Kaye

  4. Defense Science & Technology Laboratory, Porton Down, SP40JQ, Salisbury, Wilts, U.K

    Virginia Foot

  5. U. S. Army Research Laboratory, 20783-1197, Adelphi, Maryland, USA

    Ronald G. Pinnick & Steven C. Hill

  6. Nanohmics Inc., 6201 East Oltorf Street, 78741, Austin, TX, USA

    Michael W. Mayo

  7. U. S. Army Edgewood Chemical, Biological Center, Aberdeen Proving Ground, 21010, Maryland, USA

    Jerold R. Bottiger

Authors
  1. Yong-Le Pan
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  2. Jay D. Eversole
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  3. Paul H. Kaye
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  4. Virginia Foot
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  5. Ronald G. Pinnick
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  6. Steven C. Hill
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  7. Michael W. Mayo
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  8. Jerold R. Bottiger
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  9. Alan Huston
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  10. Vasanthi Sivaprakasam
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  11. Richard K. Chang
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Editor information

Editors and Affiliations

  1. University of Amsterdam, The Netherlands

    Alfons Hoekstra

  2. Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia

    Valeri Maltsev

  3. Army Research Laboratory, Adelphi, MD, U.S.A., University of Amsterdam, The Netherlands

    Gorden Videen

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© 2007 Springer

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Pan, YL. et al. (2007). BIO-AEROSOL FLUORESCENCE. In: Hoekstra, A., Maltsev, V., Videen, G. (eds) Optics of Biological Particles. NATO Science Series, vol 238. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5502-7_4

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