Abstract
Liposomes, synthetic phospholipid bilayer vesicles, have been widely used in immunology and microbiology as model membranes, drug carriers and for other purposes. They are also available as analytical tools for immunological reactions. Elegant methods based on complement-dependent liposome immune lysis for studying the interaction of antibodies with various lipid antigens have been devised by several investigators (Kinsky, 1972; Humphries and McConnell, 1974; Six et al., 1973; Smolarsky et al., 1977; Uemura and Kinsky, 1972; Uemura et al., 1980).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chan, M. W., Tan, C.-T. and Hsia, J. C. (1978). Spin membrane immunoassay; simplicity and specificity. J. Immunol. Methods, 21, 185–195.
Cole, F. X., MacDonnell, P., Myles, A., Law, S.-J., Braman, J., Dowden, D., Broeze, J., Clough, K. M. and Vovis, G. F. (1983). EMIA — a novel homogeneous phase enzyme immunoassay. In Avrames, S. (ed.), Immuno enzymatic Techniques, Elsevier Science Publishers, pp. 257–260.
Freytag, J. W. and Litchfield, W. J. (1984). Liposome-mediated immunoassay for small haptens (digoxin) independent of complement. J. Immunol. Methods, 70, 133–140.
Haga, M., Itagaki, H., Sugawara, S. and Okano, T. (1980). Liposome immunosensor for theophylline. Biochem. Biophys. Res. Comm., 95, 187–188.
Hashimoto, Y., Endoh, H. and Sugawara, M. (1984). Chemical methods for the modification of liposomes with proteins or antibodies. In Gregoriadis, G. (ed.), Liposome Technology III; Targeted Drug Delivery and Biological Interaction, CRC Publications, Boca Raton, Florida, pp. 41–49.
Hsia, J. C. and Tan, C. T. (1978). Membrane immunoassay: principle and applications of spin membrane immunoassay. Ann. NY. Acad. Sci., 308, 139–146.
Huang, L., Huang, A. and Kennel, S. J. (1984). Coupling of antibodies with liposomes. In Gregoriadis, G. (ed.), Liposome Technology III: Targeted Drug Delivery and Biological Interaction, CRC Publications, Boca Raton, Florida, pp. 51–61.
Humphries, G. K. and McConnell, H. M. (1974). Immune lysis of liposomes and erythrocyte ghosts loaded with spin label. Proc. Natl. Acad. Sci. USA, 71, 1961–1964.
Ishimori, Y., Yasuda, T., Tsumita, T., Notsuri, M., Koyama, M. and Tadakuma, T. (1984). Liposome immune lysis assay (LILA); a simple method to measure anti-protein antibody using protein antigen-bearing liposomes. J. Immunol. Methods, 75, 351–360.
Jou, Y.-H. and Bankert, R. B. (1985). Coated cells and their use. U.S. Patent No. 4, 529, 712.
Kinsky, S. C. (1972). Antibody-complement interaction with lipid model membrane. Biochem. Biophys. Acta, 265, 1–23.
Leserman, L. D., Machy, P. and Barbet, J. (1984). Covalent coupling of monoclonal antibodies and protein A to liposomes; specific interaction with cells in vitro and in vivo. In Gregoriadis, G. (ed.), Liposome Technology III: Targeted Drug Delivery and Biological Interaction, CRC Publications, Boca Raton, Florida, pp. 29–40.
Litchfield, W. J., Freytag, J. W. and Adamich, M. (1984). Highly sensitive immunoassays based on use of liposomes without complement. Clin. Chem., 30, 1441–1445.
Mandle, R. M. and Wong, Y. N. (1983). Chemiluminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer. U.S. Patent No. 4, 372, 745.
Martin, F. J. and Kung, V. T. (1985). Binding characteristics of antibody-bearing liposomes. Ann. N. Y. Acad. Sci., 446, 443–456.
Miyai, K. (1985). Advances in nonisotopic immunoassay. Adv. Clin. Chem., 24, 61–110.
O’Connell, J. P., Campbell, R. L., Fleming, B. M., Mercolino, T. J., Johnson, M. D. and McLaurin, D. A. (1985). A highly sensitive immunoassay system involving antibody-coated tubes and liposome-entrapped dye. Clin. Chem., 31, 1424–1426.
Rosenquist, E. and Vistnes, A. I. (1977). Immune lysis of spin label loaded liposomes incorporating cardiolipin; a new sensitive method for detecting anti-car diolipin antibodies in syphilis serology. J. Immunol. Methods, 15, 147–155.
Six, H. R., Uemura, K. and Kinsky, S. C. (1973). Effect of immunoglobulin class and affinity on the initiation of complement-dependent damage to liposomal model membranes sensitized with dinitrophenylated phospholipids. Biochemistry, 12, 4003–4011.
Smolarsky, M., Teiltelbaum, D., Sela, M. and Gitler, C. (1977). A simple fluorescent method to determine complement-mediated liposome immune lysis. J. Immunol. Methods, 15, 255–265.
Szoka, F. C. (1984). Liposomes with glycolipid-linked antibodies. U.S. Patent No. 4, 483, 929.
Szoka, F. C. and Papahadjopoulos, D. (1978). Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc. Natl Acad. Sci. USA, 75, 4194–4198.
Tan, C. T., Chan, S. W. and Hsia, J. C. (1981). Membrane immunoassay; a spin membrane immunoassay for thyroxine. Methods in Enzymology, 74, 152–161.
Uemura, K. and Kinsky, S. C. (1972). Active vs. passive sensitization of liposomes toward antibody and complement by dinitrophenylated derivatives of phosphatidylethanolamine. Biochemistry, 11, 4085–4094.
Uemura, K., Yuzawa-Watanabe, M., Kitagawa, N. and Taketomi, T. (1980). Liposome agglutination and liposomal membrane immune-damage assays for the characterization of antibodies to glycosphingolipids. J. Biochem., 87, 1641–1648.
Umezawa, Y., Sofue, S. and Takamoto, Y. (1982). Liposome immunoelectrode using glucose enzyme electrode. Denki Kagaka, 50, 107–108.
Editor information
Editors and Affiliations
Copyright information
© 1988 S. B. Pal and the Contributors
About this chapter
Cite this chapter
Monji, N., Cole, CA., Castro, A. (1988). Application of Liposomes in Non-isotopic Immunoassays: A Review. In: Pal, S.B. (eds) Reviews on Immunoassay Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-10318-8_5
Download citation
DOI: https://doi.org/10.1007/978-1-349-10318-8_5
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-1-349-10320-1
Online ISBN: 978-1-349-10318-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)