Abstract
The actin molecule is one of nature’s most remarkable objects: its very ubiquity throughout eukaryotic biology and the tenacity of its structure in the face of aeons of evolutionary adaptation bespeak a fundamental functional characteristic that invites our curiosity. Actin stands at the crossroads where eukaryotes first diverged from our unicellular prokaryotic ancestors, a point when cells learned to replicate and package their genomic material and to divide into daughter cells. In the structural timescape, it was time to abandon rigid cell-walls and simple flagellar-based motility, and to invest the cytoplasm with the vast new organising potential afforded by the actin molecule. The essential utility of the actin molecule for the cyto-architect is that it can spontaneously assemble into long flexible filaments, from which bundles, cables and mesh-works can be constructed. Through such extended structures forces can be transmitted, sieves and boundaries assembled, and cell membranes buttressed. Add to this the capacity to alter in a controlled manner the pattern of interconnections and the cell acquires the means to move, change shape and perform mechanical work. It is our view that, once the actin molecule emerged at the dawn of the eukaryotes as a universal mechanochemical coupling unit, its structure remained virtually unaltered throughout evolution. Instead, other actin binding proteins evolved capable of modifying and controlling the behaviour of actin to meet new functional requirements.
Preview
Unable to display preview. Download preview PDF.
References
Barden, J. A. and dos Remedios, G. G. (1984): The environment of the high-affinity cation binding site on actin and the separation between cation and ATP sites as revealed by proton NMR and fluorescence spectroscopy. J. Biochem., Tokyo, 96, 913–921
Botstein, D. (in conversation, 1985)
Chothia, C. (in conversation, 1984)
dos Remedios, C. G., Miki, M. and Barden, J. (1987). Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluation. J. Muscle Res. Cell Motil., 8, 97–117
Egelman, E. (1985). The structure of the actin thin filament (a review). J. Muscle Res. Cell Motil., 6, 129–151
Egelman, E. H., Frances, N. and DeRosier, D. J. (1982). F-actin is a helix with a random variable twist. Nature, 298, 131–135
Estes, J. E., Seiden, L. A. and Gershman, L. C. (1987). Tight binding of divalent cations to monomeric actin. J. Biol. Chem., 262, 4952–4957.
Frieden, C. (1982). The Mg++-induced conformational change in rabbit skeletal muscle G-actin. J. Biol. Chem., 257, 2882–2886
Gershman, L. C., Seiden, L. A. and Estes, J. E. (1986). High affinity binding of divalent cation to actin monomer is much stronger than previously reported. Biochem. Biophys. Res. Commun., 135, 607–614
Hambly, B. D., Barden, J. A., Miki, M. and dos Remedios, C. G. (1986). Structural and functional domains on actin. Bioessays, 4, 124–128
Harrison, S. C., Olson, A.J., Schutt, C. E., Winkler, F. K. and Bricogne, G. (1978). Tomato bushy stunt virus at 2.9 angstroms resolution. Nature, 276, 368–373
Hiromi, Y. and Hotta, Y. (1987). Actin gene mutations in Drosophila; heat shock activation in the indirect flight muscles. EMBO Jl, 4, 1681–1687
Jacobson, G. and Rosenbusch, J. (1976). ATP binding to a protease-resistant core of actin. Proc. Natl Acad. Sci. USA, 73, 2742–2746
Kabsch, W., Mannherz, H. G. and Suck, D. (1985). Three-dimensional structure of the complex of actin and DNase I at 4.5 Å resolution. EMBO Jl, 4, 2113–2118
Karlik, C. C., Coutu, M. D. and Fyrberg, E. A. (1984). A nonsense mutation within the Act88F actin gene disrupts myofibril formation in Drosophila indirect flight muscles. Cell, 38, 711–719
Konno, K. (1988). G-Actin structure revealed by chymotryptic digestion. J. Biochem., 103, 386–392
Korn, E. D. (1982). Actin polymerization and its regulation by proteins from non-muscle cells. Physiol. Rev., 62, 672–725
Korn, E. D., Carlier, M.-F. and Pantaloni, D. (1988). Actin polymerization and ATP hydrolysis. Science, N.Y., 238, 638–644
Leavitt, J., Bushar, G., Kakunaga, T., Hamada, H., Hirakawa, T., Goldman, D. and Merril, C. (1982). Variations in expression of mutant? actin accompanying incremental increases in human fibroblast tumorigenicity. Cell, 28, 259–268
Lindberg, U., Schutt, C. E., Hellsten, E., Tjader, A.-C. and Hult, T. (1988). The use of poly-L-proline sepharose in the isolation of profilin and profilin-actin complexes. Biophys. Biochim. Acta., 967, 391–400
Martin, Debra J. and Rubenstein, Peter A. (1987). Alternate pathways for removal of the class II actin initiator methionine. J. Biol. Chem., 262, 6350–6356
Miki, M. and Wahl, P. (1985). Fluorescence energy transfer between points in G-actin: the nucleotide-binding site, the metal-binding site and Cys-373 residue. Biochim. Biophys. Acta, 828, 188–195
Moir, A. J. G. and Levine, B. A. (1986). Protein cognitive sites on the surface of actin. A proton NMR study. J. Inorg. Biochem., 28, 271–278
Mornet, D. and Ue, K. (1984). Proteolysis and structure of skeletal muscle actin. Proc. Natl Acad. Sci. USA, 82, 3680–3684
Nowak, E., Strzelecka-Golaszewka, H. and Goody, R. (1988). Kinetics of nucleotide and metal ion interaction with G-actin. Biochemistry, 27, 1785–1792
Olson, A. J. (1984). Tomato Bushy Stunt Virus (a film), Palmer Film Studios, 1475 Old Country Road, Belmont, CA 94002, USA
Oosawa, F. (1983). Macromolecular assembly of actin. In Stracher, A. (Ed.), Muscle and Nonmuscle Motility, Vol. 1. Academic Press, New York, London, pp. 151–211
Pollard, T. D. (1986). Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments. J. Cell Biol., 103, 2747–2754
Pollard, T. D. and Cooper, J. A. (1986). Actin and actin-binding proteins: A critical evaluation of mechanisms and functions. Ann. Rev. Biochem., 55, 987–1035
Ponder, J. W. and Richards, F. M. (1987). Tertiary templates for proteins: use of packing criteria in the enumeration of allowed sequences for different structural classes. J. Mol. Biol., 193, 775–791
Sakabe, N., Sakabe, K., Saski, K., Kondo, H., Ema, T., Kamiya, N. and Matsushima, M. (1983). Structure of actin-DNase I at low resolution. J. Biochem., 93, 299–302
Sarma, R., Ramirez, F., Narayanan, P., McKeever, B. and Marecek, J. F. (1979). Molecular structure of a stable 2,4-dinitrophenoxide complex. A model for histidine and N-τ-methylhistidine coordination in MgATPase and their uncouplers. J. Am. Chem. Soc., 101 (17), 5015–5019
Schutt, C. (1987). Movement on the Aufbaubahn. Nature, 325, 757–758
Schutt, C. E., Strauss, N., Morikawa, K. and Lindberg, U. (1986). Crystallographic studies on the profilin: actin complex. In Yanagida, T. (Ed.), Actin: Structure and Functions. University of Tokyo Press, pp. 10–17
Schutt, C. E., Lindberg, U., Myslik, J. and Strauss, N. (1989). Molecular packing in profilin: actin crystals and its implications, J. Mol. Biol., in press
Sigler, P. (in conversation, 1985)
Solomon, Larry R. and Rubenstein, Peter A. (1987). Studies on the role of actin’s N-τ-methylhistidine using oligodeoxynucleotide-directed site-specific mutagenesis. J. Biol. Chem., 262, 11382–11388
Stock, A., Wylie, D. C., Mottonen, J. M., Lupas, A. N., Ninfa, E. G., Ninfa, A. J., Schutt, C. E. and Stock, J. B. (1988). Phospho-proteins involved in bacterial signal transduction. Cold
Spring Harbor Symposium on Quantitative Biology on Sensory Transduction., 53, 49–57
Stossel, T. P., Chaponnier, C., Ezzell, R. M., Hartwig, J. H., Janmey, P. A., Kwiatkowski, D. H., Lind, S. E., Smith, D. B., Southwick, F. S., Yin, H. L. and Zaner, K. S. (1985). Nonmuscle actin-binding proteins. Ann. Rev. Cell Biol., 1, 353–402
Suck, D., Kabsch, W. and Mannherz, H. G. (1981). Three-dimensional structure of the complex of skeletal muscle actin and bovine pancreatic DNaseI at 6 Å resolution. Proc. Natl Acad. Sci. USA, 78, 4319–4323
Sussman, D. J., Sellers, J. R., Flicker, P., Laie, E. Y., Cannon, L. E., Szent-Gyorgyi, A. G. and Fulton, C. (1984). Actin of Naegleria gruberi. J. Biol. Chem., 259, 7349–7354
Sutoh, K. (1984). Actin-actin and actin-deoyribonuclease I contact sites in the actin sequence. Biochemistry, 23, 1942–1946
Vandekerckove, J., Leavitt, J., Kakunaga, T. and Weber, K. (1980). Coexpression of a mutant γ-actin and two normal β- and γ-cytoplasmic actins in a stably transformed human cell line. Cell, 22, 893–899
Wang, Y. (1985). Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling. J. Cell Biol., 101, 597–602
Weeds, A. (1982). Actin-binding proteins—regulators of cell architecture and motility. Nature, 296, 811–815
Wegner, A. (1976). Head to tail polymerization of actin. J. Mol. Biol., 108, 139–147
Zimmerle, C. T., Patane, K. and Frieden, C. (1987). Divalent cation binding to the high- and low-affinity sites on G-actin. Biochemistry, 26, 6542–6552
Editor information
Editors and Affiliations
Copyright information
© 1990 The editor and contributors
About this chapter
Cite this chapter
Schutt, C.E., Lindberg, U. (1990). The Nature of the Actin Molecule. In: Squire, J.M. (eds) Molecular Mechanisms in Muscular Contraction. Topics in Molecular and Structural Biology. Palgrave, London. https://doi.org/10.1007/978-1-349-09814-9_2
Download citation
DOI: https://doi.org/10.1007/978-1-349-09814-9_2
Publisher Name: Palgrave, London
Print ISBN: 978-1-349-09816-3
Online ISBN: 978-1-349-09814-9
eBook Packages: EngineeringEngineering (R0)