Abstract
Trichoderma is one of the most widely used biological control agents worldwide. In addition to protecting crops through various mechanisms such as competition for nutrients and space, mycoparasitism, antibiotic production, and induction of the systemic immune response in plants, it is widely recognized for its beneficial effects on crops through the development of plants by increasing the availability of nutrients and promoting growth and development of the root system, as well as the degradation of synthetic pesticides. The most used form for its application in the field is spores, which can be produced by solid-state fermentation systems. This characteristic allows the revalorization of agro-industrial residues as a substrate in the production processes of this biological control agent, contributing to the reduction of production costs as well as the mitigation of the sources of contamination represented by the disposal of these wastes in the fields. This protocol establishes the guidelines to follow for the evaluation of the physicochemical properties of an agro-industrial residue for its use as a support in solid-state fermentation processes, as well as for its revalorization through its use in the production of Trichoderma spores for its use as a biological control agent through solid-state fermentation processes.
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Acknowledgments
Saldaña-Mendoza thanks the National Council of Science and Technology, Mexico (CONACYT), for the financial support given to his PhD studies at DIA-FCQ/UADEC.
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Saldaña-Mendoza, S.A., Chavez-González, M.L., Aguilar Gonzalez, C.N. (2023). Protocol for the Production of Trichoderma Spores for Use as a Biological Control Agent Through the Revalorization of Agro-industrial Waste. In: Aguilar Gonzalez, C.N., Gómez-García, R., Kuddus, M. (eds) Food Waste Conversion. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3303-8_12
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DOI: https://doi.org/10.1007/978-1-0716-3303-8_12
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