Abstract
Transposable elements (TEs) are abundant in the genome, and specific insertions may be co-opted to act as coding or noncoding functional elements. CRISPR-based genome editing technologies enable functional studies of TE insertions in cell lines. Here, we describe the use of CRISPR-Cas9 to create and validate genetic knockouts of TEs in mammalian cell lines.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Simpson, D.M., Chuong, E.B. (2023). Genetic Knockout of TE Insertions by CRISPR-Cas9. In: Branco, M.R., de Mendoza Soler, A. (eds) Transposable Elements. Methods in Molecular Biology, vol 2607. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2883-6_17
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DOI: https://doi.org/10.1007/978-1-0716-2883-6_17
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2882-9
Online ISBN: 978-1-0716-2883-6
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