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Immunoprecipitation of HDAC6 and Interacting Proteins

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HDAC/HAT Function Assessment and Inhibitor Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2589))

Abstract

The lysine deacetylase HDAC6 has unique structural and functional properties: It contains tandem catalytic domains that can deacetylate a variety of proteins and a zinc finger domain that binds ubiquitin. HDAC6 has been implicated in a variety of biological processes, normal or pathological, such as cellular motility, stress response, cancer, neurodegeneration, or viral infection. Due to this, HDAC6 is considered an attractive therapeutic target, and there is a major interest to identify small molecule inhibitors. To gain a mechanistic understanding of how HDAC6 impacts these different biological processes, there is a continued need to discover additional substrates as well as interacting proteins in different paradigms. One approach to achieve this is to perform HDAC6 immunoprecipitations to identify partner proteins. We describe here our optimized protocols to immunoprecipitate HDAC6 with the goal to identify or validate interacting proteins.

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Authors and Affiliations

  1. Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

    Longlong Wang, Jacint Sanchez, Daniel Hess & Patrick Matthias

  2. Faculty of Sciences, University of Basel, Basel, Switzerland

    Patrick Matthias

Authors
  1. Longlong Wang
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  2. Jacint Sanchez
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  3. Daniel Hess
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  4. Patrick Matthias
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Corresponding author

Correspondence to Patrick Matthias .

Editor information

Editors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Wang, L., Sanchez, J., Hess, D., Matthias, P. (2023). Immunoprecipitation of HDAC6 and Interacting Proteins. In: Krämer, O.H. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 2589. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2788-4_32

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  • DOI: https://doi.org/10.1007/978-1-0716-2788-4_32

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2787-7

  • Online ISBN: 978-1-0716-2788-4

  • eBook Packages: Springer Protocols

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