Abstract
Droplet-based single-cell RNA-seq (scRNA-seq) requires the addition of bar codes that mark the cell and transcript of origin. Cell-specific bar codes, typically added by cDNA elongation on beads, label each transcript derived from an individual cell. Transcript-specific bar codes serve as unique molecular identifiers and allow for the maintenance of transcript proportions after PCR-based amplification of cDNA. This chapter provides methods for generating bar-coded scRNA-seq libraries after droplet encapsulation.
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Babcock, B., Weir, S. (2023). scRNA-seq for Microcephaly Research [II]: Preparation of Single-Cell cDNA Libraries. In: Gershon, T. (eds) Microcephaly. Methods in Molecular Biology, vol 2583. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2752-5_9
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DOI: https://doi.org/10.1007/978-1-0716-2752-5_9
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2752-5
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