Abstract
Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.
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Acknowledgement
This work was supported by the National Institutes of Health (NIH) Distinguished Scholars Program (DSP) to LMR and the Division of Intramural Research (DIR) of the National Institute on Minority Health and Health Disparities (NIMHD) at NIH (1ZIAMD000016 and 1ZIAMD000018).
This work was supported by the FAPA project (PVI0122029) from Universidad de Los Andes, granted to A.V.A.
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Blancard, M., Fetterman, K.A., Burridge, P.W. (2022). Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging. In: Yan, Q. (eds) Pharmacogenomics in Drug Discovery and Development. Methods in Molecular Biology, vol 2547. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2573-6_10
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DOI: https://doi.org/10.1007/978-1-0716-2573-6_10
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2572-9
Online ISBN: 978-1-0716-2573-6
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