Abstract
Tailoring the properties of extracellular matrix hydrogels by conjugating with synthetic polymers is an emerging strategy for designing extracellular matrix-based hybrid hydrogels. This protocol provides a method for preparing a hydrogel formulation of porcine cholecystic extracellular matrix for tissue engineering applications. Here, poly(ethylene glycol) diacrylate, a synthetic polymer, was conjugated with porcine cholecystic extracellular matrix, and a biosynthetic hydrogel was prepared to have tunable physico-mechanical properties, as required for tissue engineering.
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Acknowledgments
Ms. Reshmi acknowledges the support from Kerala State Council for Science, Technology and Environment (KSCSTE—021/FSHP-LSS/2014) for the research fellowship. Dr. C. Purnima acknowledges the fellowship received from the Indian Council of Medical Research (ICMR 2020-6969).
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Raj, R., Purnima, C., Anilkumar, T.V. (2022). Preparation of a Hybrid Biosynthetic Hydrogel of Porcine Cholecystic Extracellular Matrix for Tissue Engineering Applications. In: Kumar, N., Kumar, V., Shrivastava, S., Gangwar, A.K., Saxena, S. (eds) Tissue Scaffolds. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2425-8_32
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DOI: https://doi.org/10.1007/978-1-0716-2425-8_32
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