Abstract
Most people infected by the SARS-CoV-2 virus which causes COVID-19 disease experience mild or no symptoms. Severe forms of the disease are often marked by a hyper-inflammatory response known as a cytokine storm. Thus, biomarker tests which can identify these patients and place them on the appropriate treatment regime at the earliest possible phase would help to improve outcomes. Here we describe an automated microarray-based immunoassay using the Fraunhofer lab-on-a-chip platform for analysis of C-reactive protein due to its role in the hyper-inflammatory response.
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References
Kannan S, Shaik Syed Ali P, Sheeza A et al (2020) COVID-19 (Novel Coronavirus 2019) – recent trends. Eur Rev Med Pharmacol Sci 24(4):2006–2011
Wang J, Jiang M, Chen X, Montaner LJ (2020) Cytokine storm and leukocyte changes in mild versus severe SARS-CoV-2 infection: review of 3939 COVID-19 patients in China and emerging pathogenesis and therapy concepts. J Leukoc Biol 108(1):17–41
Ballow M, Haga CL (2021) Why do some people develop serious COVID-19 disease after infection, while others only exhibit mild symptoms? J Allergy Clin Immunol Pract 9(4):1442–1448
Cyprian F, Sohail MU, Abdelhafez I et al (2021) SARS-CoV-2 and immune-microbiome interactions: lessons from respiratory viral infections. Int J Infect Dis 105:540–550
Van Vo G, Bagyinszky E, Park YS et al (2021) SARS-CoV-2 (COVID-19): beginning to understand a new virus. Adv Exp Med Biol 1321:3–19
Bandeira IP, Schlindwein MAM, Breis LC et al (2021) Neurological complications of the COVID-19 pandemic: what have we got so far? Adv Exp Med Biol 1321:21–31
Mezoh G, Crowther NJ (2021) Endothelial dysfunction as a primary consequence of SARS-CoV-2 infection. Adv Exp Med Biol 1321:33–43
Abiri B, Guest PC, Vafa M (2021) Obesity and risk of COVID-19 infection and severity: available evidence and mechanisms. Adv Exp Med Biol 1321:97–107
George JA, Mayne ES (2021) The novel coronavirus and inflammation. Adv Exp Med Biol 1321:127–138
Wiggill TM, Mayne ES, Vaughan JL (2021) Overview of the haematological effects of COVID-19 infection. Adv Exp Med Biol 1321:163–172
Louw S, Jacobson BF, Mayne ES et al (2021) The novel coronavirus and haemostatic abnormalities: pathophysiology, clinical manifestations, and treatment recommendations. Adv Exp Med Biol 1321:173–180
Jafari-Oori M, Ghasemifard F, Ebadi A et al (2021) Acute respiratory distress syndrome and COVID-19: a scoping review and meta-analysis. Adv Exp Med Biol 1321:211–228
Zhao Y, Qin L, Zhang P et al (2020) Longitudinal COVID-19 profiling associates IL-1RA and IL-10 with disease severity and RANTES with mild disease. JCI Insight 5(13):e139834. https://doi.org/10.1172/jci.insight.139834
Spadaro S, Fogagnolo A, Campo G et al (2021) Markers of endothelial and epithelial pulmonary injury in mechanically ventilated COVID-19 ICU patients. Crit Care 25(1):74. https://doi.org/10.1186/s13054-021-03499-4
Liu BM, Martins TB, Peterson LK et al (2021) Clinical significance of measuring serum cytokine levels as inflammatory biomarkers in adult and pediatric COVID-19 cases: a review. Cytokine 142:155478. https://doi.org/10.1016/j.cyto.2021.155478
Syed F, Li W, Relich RF et al (2021) Excessive matrix metalloproteinase-1 and hyperactivation of endothelial cells occurred in COVID-19 patients and were associated with the severity of COVID-19. J Infect Dis 224(1):60–69
Egorov AI, Griffin SM, Fuzawa M et al (2021) A multiplex noninvasive salivary antibody assay for SARS-CoV-2 infection and its application in a population-based survey by mail. Microbiol Spectr Sep 15:e0069321. https://doi.org/10.1128/Spectrum.00693-21. Online ahead of print
Schumacher S, Nestler J, Otto T, Wegener M, Ehrentreich-Förster E, Michel D et al (2012) Highly-integrated lab-on-chip system for point-of-care multiparameter analysis. Lab Chip 12:464–473
Schumacher S, Ludecke C, Ehrentreich-Förster E, Bier FF (2013) Platform technologies for molecular diagnostics near the patient’s bedside. Adv Biochem Eng Biotechnol 133:75–87
Streit P, Nestler J, Shaporin A, Schulze R, Gessner T (2016) Thermal design of integrated heating for lab-on-a-chip systems. In: Proceedings of the 17th international conference on thermal, mechanical and multi-physics simulation and experiments in microelectronics and microsystems (EuroSimE), April 18–20, pp 1–6
Potempa LA, Rajab IM, Hart PC et al (2020) Insights into the use of C-reactive protein as a diagnostic index of disease severity in COVID-19 infections. Am J Trop Med Hyg 103(2):561–563
Liu F, Li L, Xu M et al (2020) Prognostic value of interleukin-6, C-reactive protein, and procalcitonin in patients with COVID-19. J Clin Virol 127:104370. https://doi.org/10.1016/j.jcv.2020.104370
Terpos E, Ntanasis-Stathopoulos I, Elalamy I et al (2020) Hematological findings and complications of COVID-19. Am J Hematol 95(7):834–847
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Peter, H., Mattig, E., Guest, P.C., Bier, F.F. (2022). Lab-on-a-Chip Immunoassay for Prediction of Severe COVID-19 Disease. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 2511. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2395-4_17
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DOI: https://doi.org/10.1007/978-1-0716-2395-4_17
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