Abstract
Apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) is an adaptor protein that is essential for the activation of several inflammasome complexes. Activation of inflammasomes leads to pathogenic clearance and inflammatory cell death called pyroptosis. Upon inflammasome activation, ASC oligomerization leads to the recruitment and activation of caspase-1, which in turn converts pro-inflammatory cytokines (e.g., pro-IL-1β, pro-IL-18) to their mature active form. Given its central role in inflammasome activation, ASC oligomerization is used as an indicator of inflammasome activation. Here we describe how ASC oligomerization can be detected by Western blotting.
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References
Abdul Sater A, Philpott D (2016) Inflammasomes. In: Encyclopedia of immunobiology. Elsevier Science
Lu A, Magupalli VG, Ruan J et al (2014) Unified polymerization mechanism for the assembly of ASC-dependent inflammasomes. Cell 156:1193–1206
Tweedell RE, Kanneganti T-D (2020) Advances in inflammasome research: recent breakthroughs and future hurdles. Trends Mol Med 26:969–971
Acknowledgments
This work was supported by grants received from the Arthritis Society and the Canadian Institute for Health Research (CIHR).
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Zangiabadi, S., Akram, A., Abdul-Sater, A.A. (2022). Detection of ASC Oligomerization by Western Blotting. In: Abdul-Sater, A.A. (eds) The Inflammasome. Methods in Molecular Biology, vol 2459. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2144-8_7
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DOI: https://doi.org/10.1007/978-1-0716-2144-8_7
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2144-8
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