Abstract
The N-terminomics approach of Terminal Amine Isotopic Labeling of Substrates (TAILS) enables the identification and quantification of natural and neo-N-termini of proteins using liquid chromatography and tandem mass spectrometry (LC-MS/MS). This methodology has been used to study protease function and identify protease substrates in cell culture systems, animal disease models, and more recently, has been applied to clinical samples. Here, we present the application of TAILS to tissue and liquid biopsies.
Anthonia Anowai, Sameeksha Chopra, Barbara Mainoli, Daniel Young are Co-first authors (alphabetical)
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References
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Acknowledgements
The TAILS methodology was first developed in the laboratory of Chris Overall and published in 2010 [2, 3]. This work was supported by Natural Sciences and Engineering Research Council (NSERC) Discovery Grant (DGECR-2019-00112), NSERC Tri-Council Masters Scholarship (A.A.), Cumming School of Medicine Graduate Scholarship (A.A), UCalgary Faculty of Graduate Studies Masters Research scholarship (A.A.), the R.R Singleton Summer Studentship (S.C.).
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Anowai, A., Chopra, S., Mainoli, B., Young, D., Dufour, A. (2022). N-Terminomics/TAILS of Tissue and Liquid Biopsies. In: Geddes-McAlister, J. (eds) Proteomics in Systems Biology. Methods in Molecular Biology, vol 2456. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2124-0_7
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DOI: https://doi.org/10.1007/978-1-0716-2124-0_7
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