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Production of Laccases from Agricultural Wastes: Strain Isolation and Selection, Enzymatic Profiling, and Lab-Scale Production

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Mycoremediation Protocols

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Abstract

Fungal laccases (copper-containing oxidases) have profound applications in bioremediation and various other industrial and biotechnological areas. This chapter outlines recent developments in laccase production technologies, with a focus on the selection of the most promising producing fungal species, general considerations on media components and culture conditions, lab-scale production, and partial purification of enzymes. Furthermore, protocols related to solid-state fermentation (utilizing agriculture wastes), such as one-step affinity chromatography (employing a mesoporous material as support), and electrophoresis (used to check the enzyme purity), are described.

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Authors and Affiliations

  1. ITANOA–CONICET, EEAOC, Las Talitas, Tucumán, Argentina

    Pablo M. Ahmed

  2. PROIMI–CONICET, SM de Tucumán, Tucumán, Argentina

    Hipólito F. Pajot & Pablo M. Fernández

  3. FACEN, UNCA, SFV de Catamarca, Catamarca, Argentina

    Hipólito F. Pajot & Pablo M. Fernández

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  1. Pablo M. Ahmed
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  2. Hipólito F. Pajot
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  3. Pablo M. Fernández
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Editors and Affiliations

  1. Department of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Pitipana, Homagama, Sri Lanka

    Dhanushka Udayanga

  2. Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China

    Pankaj Bhatt

  3. Department of Botany, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka

    Dimuthu Manamgoda

  4. Universidad Nacional de Tucumán, Tucumán, Argentina

    Juliana Maria Saez

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Ahmed, P.M., Pajot, H.F., Fernández, P.M. (2022). Production of Laccases from Agricultural Wastes: Strain Isolation and Selection, Enzymatic Profiling, and Lab-Scale Production. In: Udayanga, D., Bhatt, P., Manamgoda, D., Saez, J.M. (eds) Mycoremediation Protocols. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2006-9_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2006-9_12

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