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Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells

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Human Naïve Pluripotent Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2416))

Abstract

Generating patient-specific stem cells representing the onset of development has become possible since the discovery of somatic cell reprogramming into induced pluripotent stem cells. However, human pluripotent stem cells are generally cultured in a primed pluripotent state: they are poised for differentiation and represent a stage of development corresponding to post-implantation epiblast. Here, we describe a protocol to reprogram human fibroblasts into naive pluripotent stem cells by overexpressing the transcription factors OCT4, SOX2, KLF4, and c-MYC using Sendai viruses. The resulting cells represent an earlier stage of development that corresponds to pre-implantation epiblast. We also discuss validation methods for human naive pluripotent stem cells.

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Author notes

  1. Constance Onfray and Jia Ping Tan contributed equally to this work.

Authors and Affiliations

  1. Université de Nantes, CHU Nantes, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, F-44000 Nantes, France, Nantes, France

    Constance Onfray, Stéphanie Kilens & Laurent David

  2. Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia

    Jia Ping Tan, Xiaodong Liu & Jose Polo

  3. Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Clayton, VIC, Australia

    Jia Ping Tan, Xiaodong Liu & Jose Polo

  4. Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia

    Jia Ping Tan, Xiaodong Liu & Jose Polo

  5. Université de Nantes, CHU Nantes, INSERM, CNRS, SFR Santé, FED 4203, INSERM UMS 016, CNRS UMS 3556, F-44000 Nantes, France, Nantes, France

    Laurent David

Authors
  1. Constance Onfray
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  2. Jia Ping Tan
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  3. Stéphanie Kilens
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  4. Xiaodong Liu
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  5. Jose Polo
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  6. Laurent David
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Corresponding authors

Correspondence to Jose Polo or Laurent David .

Editor information

Editors and Affiliations

  1. Babraham Institute, Cambridge, UK

    Peter Rugg-Gunn

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Onfray, C., Tan, J.P., Kilens, S., Liu, X., Polo, J., David, L. (2022). Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells. In: Rugg-Gunn, P. (eds) Human Naïve Pluripotent Stem Cells. Methods in Molecular Biology, vol 2416. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1908-7_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1908-7_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1907-0

  • Online ISBN: 978-1-0716-1908-7

  • eBook Packages: Springer Protocols

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