Abstract
Biotechnological advancements have paved newer avenues for developing and designing novel and effective vaccines for rendering protection from various types of infectious diseases. Use of immunogenic genes via plasmid DNA constitutes an important next-generation biotechnological approach to fish immunization. In addition, the use of nanotechnology has significantly addressed the issue of mucosal mode of DNA vaccine delivery in aquaculture. Taking together both these advance technologies, this chapter entails a detailed protocol for the development of a nano-conjugated bicistronic DNA vaccine using chitosan nanoparticles as delivery vehicle, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Edwardsiella tarda as antigenic gene and interferon gamma (IFN-γ) gene of Labeo rohita as molecular adjuvant.
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References
Kumari R, Kole S, Soman P, Rathore G, Tripathi G, Makesh M, Rajendran KV, Bedekar MK (2018) Bicistronic DNA vaccine against Edwardsiella tarda infection in Labeo rohita: construction and comparative evaluation of its protective efficacy against monocistronic DNA vaccine. Aquaculture 485:201–209
Kole S, Kumari R, Anand D, Kumar S, Sharma R, Tripathi G, Makesh M, Rajendran KV, Bedekar MK (2018) Nanoconjugation of bicistronic DNA vaccine against Edwardsiella tarda using chitosan nanoparticles: evaluation of its protective efficacy and immune modulatory effects in Labeo rohita vaccinated by different delivery routes. Vaccine 36(16):2155–2165
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Bedekar, M.K., Kole, S. (2022). Development of Nano-Conjugated DNA Vaccine Against Edwardsiellosis Disease in Fish. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2411. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1888-2_11
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DOI: https://doi.org/10.1007/978-1-0716-1888-2_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1887-5
Online ISBN: 978-1-0716-1888-2
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