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Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering

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Membrane Lipids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2402))

Abstract

Lipid domains in model membranes are routinely studied to provide insight into the physical interactions that drive raft formation in cellular membranes. Using small angle neutron scattering, contrast-matching techniques enable the detection of lipid domains ranging from tens to hundreds of nanometers which are not accessible to other techniques without the use of extrinsic probes. Here, we describe a probe-free experimental approach and model-free analysis to identify lipid domains in freely floating vesicles of ternary phase separating lipid mixtures.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada

    Mitchell DiPasquale, Michael H.  L. Nguyen & Stuart R. Castillo

  2. Department of Chemistry, University of Tennessee, Knoxville, TN, USA

    Frederick A. Heberle

  3. Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada

    Drew Marquardt

  4. Department of Physics, University of Windsor, Windsor, ON, Canada

    Drew Marquardt

Authors
  1. Mitchell DiPasquale
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  2. Michael H.  L. Nguyen
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  3. Stuart R. Castillo
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  4. Frederick A. Heberle
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  5. Drew Marquardt
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Corresponding author

Correspondence to Drew Marquardt .

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Editors and Affiliations

  1. School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia

    Charles G. Cranfield

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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DiPasquale, M., Nguyen, M.H. ., Castillo, S.R., Heberle, F.A., Marquardt, D. (2022). Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering. In: Cranfield, C.G. (eds) Membrane Lipids. Methods in Molecular Biology, vol 2402. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1843-1_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1843-1_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1842-4

  • Online ISBN: 978-1-0716-1843-1

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