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Detection of Cytokine-Secreting Cells by Enzyme-Linked Immunospot (ELISpot)

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Single-Cell Protein Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2386))

Abstract

The enzyme-linked immunospot (ELISpot) is a highly sensitive immunoassay that measures the frequency of cytokine-secreting cells at the single-cell level. The secreted molecules are detected by using a detection antibody system similar to that used in the enzyme-linked immunosorbent assay (ELISA). The ELISpot assay is carried out in a 96-well plate and an automated ELISpot reader is used for analysis. The assay is easy to perform, robust and allows rapid analysis of a large number of samples and is not limited to measurement of cytokines; it is suitable for almost any secreted protein where single-cell analysis is of interest.

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Acknowledgments

We thank Jens Gertow, Bartek Makower, and Kopek Nihlmark for critical reading of the manuscript and Mabtech/Tanja Zamani for illustrations.

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Authors and Affiliations

  1. Mabtech AB, Nacka Strand, Stockholm, Sweden

    Bernt Axelsson

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  1. Bernt Axelsson
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Correspondence to Bernt Axelsson .

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  1. Mission Bio, Inc., South San Francisco, CA, USA

    Aik T. Ooi

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Axelsson, B. (2022). Detection of Cytokine-Secreting Cells by Enzyme-Linked Immunospot (ELISpot). In: Ooi, A.T. (eds) Single-Cell Protein Analysis. Methods in Molecular Biology, vol 2386. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1771-7_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1771-7_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1770-0

  • Online ISBN: 978-1-0716-1771-7

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