Abstract
Enzymes are the best available catalysts for any biological reactions emerging as alternatives to chemical technology. Proper identification of enzyme source is crucial. Although enzymes have been in human use from time immemorial their industrial applications are myriad and one such commercially important enzymes is protease. Microbes are easy to grow with well-established downstream processing methods. Among various microbes, Actinobacter is an attractive agent for such commercial production of protease. Several isolates of Actinobacter have proved successful in the production of protease from various genera such as Streptomyces, Nocardia, and Nocardiopsis. Proteases are essential for processing of leather, textile, and brewery baking industries. One important aspect is to identify, screen, and analyze the protease from Actinobacter source for commercial production. This chapter encompasses protocols involved in isolation and production of protease from Actinobacter sources.
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References
Grewal S, Mutha P (2010) Enzyme technology. Published by Agrobios (India), 145–148. ISBN 978-81-7754-388-9
Alnandi HS (2012) Isolation and screening of extracellular proteases produced by new isolated BacillusSp. J Appl Pharm Sci 2(9):071–074
Kumar CG, Takagi H (1999) Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnol Adv 17(7):561–594
Gupta R, Beg Q, Lorenz P (2002) Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol 59(1):15–32
Kamath P, Subrahmanyam VM, Venkata-Rao J, Vasantha-Raj P (2010) Optimization of cultural conditions for protease production by a fungal species. Indian J Pharm Sci 72(2):161–166
Flores-Gallegos AC, Nava-Reyna E (2019) Plant growth-promoting microbial enzymes. In: Enzymes in food biotechnology. Academic Press, New York, pp 521–534
McCarthy AJ, Peace E, Broda P (1985) Studies on the extracellular xylanase activity of some thermophilicactinomycetes. Appl Microbiol Biotechnol 21(3–4):238–244
Vuppu S, Mishra B (2011) An overview of some reported soil enzyme producing microorganisms. Indian J Fundamental Appl Life Sci 1(4):180–186
Vishweshwar R, Sreeramulu K, Akbar SM and Hari CS (2016) Isolation and Characterisation of Gut Bacterial Proteases Involved in Inducing Pathogenicity of Bacillus thurungienesis Toxin in Cotton Bollworm, Helicoverpa armigera. Frontiers in Microbiology, Microbial Physiology and Metabolism, 7: 1–11
Uyar F, Porsuk I, Kizil G, Yilma E (2011) Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. EurAsia J BioSci 5:1–9
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Ashwitha Gopal, N. Swamy, V., Arul, S., M, J. (2022). Screening of Protease from Actinobacteria. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_79
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DOI: https://doi.org/10.1007/978-1-0716-1728-1_79
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