Abstract
Actinobacteria, the Gram-positive bacteria with high G + C content, are important for nutrient recycling of natural substrates and degradation of soil organic material. Awakening their potential of cellulase production can resolve environmental demands of bioconversion of plant-based cellulosic and lignocellulosic waste as its crystalline structure and insoluble nature represent a big challenge for enzymatic hydrolysis. Actinobacteria isolated from soil are subjected to screening and identified based on staining methods and biochemical tests.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Islam F, Roy N (2018) Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses. BMC Res Notes 11(1):1–6. https://doi.org/10.1186/s13104-018-3558-4
Putri AL, Setiawan R (2019) Isolation and screening of actinomycetes producing cellulase and xylanase from Mamasa soil, West Sulawesi. IOP Conference Series: Earth and Environmental Science 308(1):5–11. https://doi.org/10.1088/1755-1315/308/1/012035
Rasul F, Afroz A, Rashid U, Mehmood S, Sughra K, Zeeshan N (2015) Screening and characterization of cellulase producing bacteria from soil and waste (molasses) of sugar industry. Int J Biosci 6655:230–238. https://doi.org/10.12692/ijb/6.3.230-238
Kumari S, Sharma U, Krishna R, Sinha K, Kumar S (2019) Screening and molecular characterization of Cellulase producing Actinobacteria from Litchi orchard. Curr Chem Biol 13(1):90–101. https://doi.org/10.2174/2212796812666180718114432
SahA S, Roy RN, Sen SK, Ray AK (2006) Characterization of cellulose producing bacteria from the digestive tract of tilapia, Oreochromis mossambica (Peters) and grass carp, Ctenopharyngodon idella (Valenciennes). Aquac Res 37:380–388. https://doi.org/10.1111/j.1365-2109.2006.01442.x
Goodfellow M (2010) Selective isolation of actinobacteria. In: Manual of industrial microbiology and biotechnology. ASM Press, Washington, DC, pp 13–27
Saini A, Aggarwal NK, Yadav A (2016) Cellulolytic potential of Actinomycetes isolated from different habitats. Bioeng Biosci 4(5):88–94. https://doi.org/10.13189/bb.2016.040503
Khatiwada P, Ahmed J, Sohag MH, Islam K, Azad AK (2016) Isolation, screening and characterization of Cellulase producing bacterial isolates from municipal solid wastes and Rice straw wastes. J Bioprocess Biotech 6:280. https://doi.org/10.4172/2155-9821.1000280
Irfan M, Safdar A, Syed Q, Nadeem M (2012) Selülaz aktivitesine sahip bakterilerin topraktan izolasyonu, deerlendirilmesi ve selülaz üretim ve aktivitesinin optimizasyonu. Turkish J Biochem 37(3):287–293. https://doi.org/10.5505/tjb.2012.09709
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Swamy, V.N., Haneen, M.A., Jayashankar, M. (2022). Screening of Cellulase from Actinobacteria. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_77
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1728-1_77
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1727-4
Online ISBN: 978-1-0716-1728-1
eBook Packages: Springer Protocols