Abstract
Lipases are enzymes that act on lipids and have the ability to catalyze the hydrolysis of triacylglycerol in glycerol and free fatty acids at the oil–water interface. These enzymes are applied in several areas, such as in the food, pharmaceutical, paper, textile, and detergent industries, among others. Similarly to other enzymes, lipases can be obtained by microbes, among which actinobacteria, also known as actinomycetes, have shown great potential for production. The purpose of this chapter is to provide an overview of recent studies on the production of lipases by actinobacteria and to present experimental protocols for the conditions of isolation and cultivation of actinobacteria, as well as the screening and determination of enzymatic activity. According to studies found in the literature, several species of actinobacteria are lipase-producing, which can be isolated from different types of environments. As regards the cultivation conditions, the characteristics of the culture medium and the specificities of the samples obtained must be taken into consideration. They vary widely in terms of temperature and incubation time, among other parameters. Finally, the determination of enzymatic activity by the spectrophotometric method is proposed employing different substrates.
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Pizarro, I.F., de Souza, H.F., dos Santos Ferreira, J., Maldonado, R.R., Kamimura, E.S., Aguiar-Oliveira, E. (2022). Production of Lipase by Actinobacteria. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_75
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DOI: https://doi.org/10.1007/978-1-0716-1728-1_75
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