Abstract
Within a bacterial population, there can be a subpopulation of cells with an antibiotic-tolerant persister phenotype characterized by long lag phase. Their long lag phase necessitates long (hours or days) periods of single-cell observation to capture high-quality quantitative information about persistence. We describe a method of single-cell imaging using glass bottom dishes and a nutrient agarose pad that allows for long-term single-cell microscopy observation in a stable environment. We apply this method to characterize the lag phase and persistence of individual Escherichia coli cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Şimşek E, Kim M (2018) The emergence of metabolic heterogeneity and diverse growth responses in isogenic bacterial cells. ISME J 12:1199–1209
Şimşek E, Kim M (2019) Power-law tail in lag time distribution underlies bacterial persistence. Proc Natl Acad Sci U S A 116:17635–17640
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Dawson, E., Şimşek, E., Kim, M. (2021). Observing Bacterial Persistence at Single-Cell Resolution. In: Verstraeten, N., Michiels, J. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 2357. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1621-5_6
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1621-5_6
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1620-8
Online ISBN: 978-1-0716-1621-5
eBook Packages: Springer Protocols