Abstract
Western blot processing is a well-established procedure that includes protein extraction from tissues and cells, gel electrophoresis separation, transfer to a membrane, and immunodetection with specific antibodies. Here, we show that optimization of washing helps to maximize the specific interactions of antigens and antibodies. Performing all washing steps at 4 °C ensures a maximal signal to noise ratio and reduces nonspecific signals.
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Acknowledgments
The experiments performed were in part supported by Precision Biosystems, LLC and Rockland Immunochemicals Inc.
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Yukhananov, R., Chimento, D.P., Marlow, L.A. (2022). Western Blot Processing Optimization: The Perfect Blot. In: Navid, A. (eds) Microbial Systems Biology. Methods in Molecular Biology, vol 2349. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1585-0_4
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DOI: https://doi.org/10.1007/978-1-0716-1585-0_4
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