Abstract
Chlorophyll is the major pigment in plants and acts as a biomass indicator of aquatic microalgae which assists food webs within the sea. The chlorophyll content is dependent on the environment and season. In phytoplankton, low chlorophyll is observed during the winter season, and this may be due to limitation of sunlight. There are some methods to estimate the content of chlorophyll, such as based on the absorption of light by aqueous acetone extracts, based on the absorbance or reflectance of certain wavelengths of light by intact leaves using handheld chlorophyll meters.
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References
Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
Lorenzen CJ (1967) Determination of chlorophyll and pheo-pigments: spectrophotometric equations. Limnol Oceanogr 12:343–346
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Senthilkumar, M., Amaresan, N., Sankaranarayanan, A. (2021). Determination of Chlorophyll. In: Plant-Microbe Interactions. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1080-0_37
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DOI: https://doi.org/10.1007/978-1-0716-1080-0_37
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1079-4
Online ISBN: 978-1-0716-1080-0
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