Abstract
MicroRNAs (miRNAs) are expressed in all cell types, including pericytes, and play essential roles in vascular development, homeostasis, and disease. Manipulation of pericytes with miRNA mimics and inhibitors represents an essential tool to study the role of pericytes in vascular development and regeneration and to better understand the therapeutic potential of miRNA manipulation in pericytes. Here we describe methods for manipulating pericyte function by using miRNA mimics and inhibitors. We also describe methods to assess pericyte function (proliferation and migration) after manipulation with miRNAs and explain how miRNA gene targets can be identified and validated in pericytes after manipulation with miRNA.
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Acknowledgments
This work was supported by the British Heart Foundation Centre for Vascular Regeneration (BHF-CVR).
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Vitiello, M., Cathcart, B., Caporali, A., Meloni, M. (2021). Manipulating Pericyte Function with MicroRNAs. In: Péault, B.M. (eds) Pericytes. Methods in Molecular Biology, vol 2235. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1056-5_10
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DOI: https://doi.org/10.1007/978-1-0716-1056-5_10
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1056-5
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