Abstract
Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.
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Acknowledgments
This work was funded by Medical Research Council grant MR/N021444/1 to L.M., by the Engineering and Physical Sciences Research Council grants EP/R041695/1 and EP/S01876X/1 to L.M., and by BrisSynBio, a BBSRC/EPSRC Synthetic Biology Research Centre (BB/L01386X/1) to L.M.
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Khazim, M., Pedone, E., Postiglione, L., di Bernardo, D., Marucci, L. (2021). A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells. In: Menolascina, F. (eds) Synthetic Gene Circuits . Methods in Molecular Biology, vol 2229. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1032-9_10
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DOI: https://doi.org/10.1007/978-1-0716-1032-9_10
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