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Arabidopsis Photosynthetic and Heterotrophic Cell Suspension Cultures

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2200))

Abstract

Cell suspension cultures represent a widely used experimental tool suitable to perform a variety of structural and physiological studies in a more simplified system compared to the organism in toto. In this chapter we describe the methods routinely used in our laboratory to establish and maintain Arabidopsis photosynthetic and heterotrophic cell suspension cultures, containing either chloroplasts or amyloplasts, respectively. The use of these in vitro systems may allow to obtain insights into the unique features of chloroplasts versus non-green plastids, as well as their integration in the structural and metabolic compartmentalization of the plant cell.

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Acknowledgments

We are grateful to R. Moscatiello and S. Sello, previous members of our laboratory (Padova, Italy), for their contribution to the establishment of the described experimental procedures. We also thank I. Szabo (Padova, Italy) for useful discussions and T. Morosinotto (Padova, Italy) for the availability of the PAM imaging apparatus. This work was supported by Progetti di Ricerca di Interesse Dipartimentale (PRID 2018) (prot. BIRD180317) and Dotazione Ordinaria della Ricerca 2018-2019 to LN.

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Authors and Affiliations

  1. Department of Biology, University of Padova, Padova, Italy

    Enrico Cortese, Luca Carraretto, Barbara Baldan & Lorella Navazio

  2. Botanical Garden, University of Padova, Padova, Italy

    Barbara Baldan & Lorella Navazio

Authors
  1. Enrico Cortese
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  2. Luca Carraretto
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  3. Barbara Baldan
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  4. Lorella Navazio
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Corresponding author

Correspondence to Lorella Navazio .

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Editors and Affiliations

  1. Centro Nacional de Biotecnología, CSIC Centro Nacional de Biotecnología, Madrid, Spain

    Jose J. Sanchez-Serrano

  2. Centro de Investigaciones Biologicas, CSIC Madrid Centro de Investigaciones Biologicas, Madrid, Spain

    Julio Salinas

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Cortese, E., Carraretto, L., Baldan, B., Navazio, L. (2021). Arabidopsis Photosynthetic and Heterotrophic Cell Suspension Cultures. In: Sanchez-Serrano, J.J., Salinas, J. (eds) Arabidopsis Protocols . Methods in Molecular Biology, vol 2200. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0880-7_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0880-7_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0879-1

  • Online ISBN: 978-1-0716-0880-7

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