Abstract
Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.
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Acknowledgments
We would like to thank the Hessen State Ministry of Higher Education, Research and the Arts for the financial support within the Hessen initiative for scientific and economic excellence (LOEWE-Program, LOEWE ZIB (Center for Insect Biotechnology and Bioresources) and LOEWE Center DRUID (Novel Drug Targets against Poverty-Related and Neglected Tropical Infectious Diseases)). The authors acknowledge Catherine Meckel-Oschmann for revising the chapter.
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Harnischfeger, J., Käßer, L., Zitzmann, J., Salzig, D., Czermak, P. (2021). Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System. In: Pfeifer, B.A., Hill, A. (eds) Vaccine Delivery Technology. Methods in Molecular Biology, vol 2183. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0795-4_8
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DOI: https://doi.org/10.1007/978-1-0716-0795-4_8
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