Abstract
A simple procedure for obtaining outer membrane vesicles from Salmonella enterica and the use of hydrogels as vaccine delivery system is described. A heat treatment in saline solution of whole bacteria rendered the release of outer membrane vesicles containing relevant antigenic components. The immunogenicity of these antigens when administered by the intranasal route may be improved after embedment into hydrogels to increase residence half-time and thus activate the mucosal immune system.
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Acknowledgments
Y.P. has held a Ph.D. scholarship from the “Asociación de Amigos” of the University of Navarra. This work was supported by a FIS grant PI16/00071, “Ministerio de Sanidad y Consumo” from Spain. The author would like to thank Monica Laires Rodrigues for assistance with hydrogel characterization .
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Pastor, Y., Ting, I., Berzosa, M., Irache, J.M., Gamazo, C. (2021). Vaccine Based on Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System. In: Schatten, H. (eds) Salmonella. Methods in Molecular Biology, vol 2182. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0791-6_14
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DOI: https://doi.org/10.1007/978-1-0716-0791-6_14
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