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Protein Structure Analysis and Validation with X-Ray Crystallography

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Protein Downstream Processing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2178))

Abstract

X-ray crystallography is the main technique for the determination of protein structures. About 85% of all protein structures known to date have been elucidated using X-ray crystallography. Knowledge of the three-dimensional structure of proteins can be used in various applications in biotechnology, biomedicine, drug design , and basic research and as a validation tool for protein modifications and ligand binding. Moreover, the requirement for pure, homogeneous, and stable protein solutions in crystallizations makes X-ray crystallography beneficial in other fields of protein research as well. Here, we describe the technique of X-ray protein crystallography and the steps involved for a successful three-dimensional crystal structure determination.

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Author information

Authors and Affiliations

  1. Turku Bioscience Centre, University of Turku and Åbo Akademi University, BioCity, Turku, Finland

    Anastassios C. Papageorgiou, Nirmal Poudel & Jesse Mattsson

Authors
  1. Anastassios C. Papageorgiou
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  2. Nirmal Poudel
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  3. Jesse Mattsson
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Corresponding author

Correspondence to Anastassios C. Papageorgiou .

Editor information

Editors and Affiliations

  1. Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece

    Nikolaos E. Labrou

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Papageorgiou, A.C., Poudel, N., Mattsson, J. (2021). Protein Structure Analysis and Validation with X-Ray Crystallography. In: Labrou, N.E. (eds) Protein Downstream Processing. Methods in Molecular Biology, vol 2178. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0775-6_25

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  • DOI: https://doi.org/10.1007/978-1-0716-0775-6_25

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0774-9

  • Online ISBN: 978-1-0716-0775-6

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