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Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.)

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Virus-Induced Gene Silencing in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2172))

Abstract

Virus-induced gene silencing (VIGS) is a transcript suppression technique that enables the functional characterization of genes in recalcitrant transformation plants. This technique consists in cloning a short fragment of a gene of interest into a viral vector, such as TRV (Tobacco rattle virus), and this viral construction is used to agro-infiltrate the plant. VIGS induces posttranscriptional gene silencing (PTGS) that results in the specific sequence degradation of target RNAs. Here we describe a VIGS protocol using the Gateway-based TRV vector for the study of genes in chili pepper plants.

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Acknowledgments

We thank Dr. María del Rocío Gómez-García and Dr. César Aza-González for their efforts in the standardization of the Gateway-based TRV VIGS protocol in chili pepper plants in our laboratory. This work was supported by a grant from Consejo Nacional de Ciencia y Tecnología (Conacyt), Mexico, project 1570.

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Authors and Affiliations

  1. Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, Gto., Mexico

    Magda Lisette Arce-Rodríguez & Neftalí Ochoa-Alejo

Authors
  1. Magda Lisette Arce-Rodríguez
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  2. Neftalí Ochoa-Alejo
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Corresponding author

Correspondence to Neftalí Ochoa-Alejo .

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Editors and Affiliations

  1. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Vincent Courdavault

  2. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Sébastien Besseau

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Arce-Rodríguez, M.L., Ochoa-Alejo, N. (2020). Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.). In: Courdavault, V., Besseau, S. (eds) Virus-Induced Gene Silencing in Plants. Methods in Molecular Biology, vol 2172. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0751-0_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0751-0_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0750-3

  • Online ISBN: 978-1-0716-0751-0

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