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Virus-Induced Gene Silencing in Olive Tree (Oleaceae)

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Virus-Induced Gene Silencing in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2172))

Abstract

Research on gene functions in non-model tree species is hampered by a number of difficulties such as time-consuming genetic transformation protocols and extended period for the production of healthy transformed offspring, among others. Virus-induced gene silencing (VIGS) is an alternative approach to transiently knock out an endogenous gene of interest (GOI) by the introduction of viral sequences encompassing a fragment of the GOI and to exploit the posttranscriptional gene silencing (PTGS) mechanism of the plant, thus triggering silencing of the GOI. Here we describe the successful application of Tobacco rattle virus (TRV)-mediated VIGS through agroinoculation of olive plantlets. This methodology is expected to serve as a fast tracking and powerful tool enabling researchers from diversified fields to perform functional genomic analyses in the olive tree.

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Acknowledgments

P.H. acknowledges funding from General Secretariat of Research and Technology, Greece, National Emblematic Action “Olive Routes.” M.T. acknowledges funding from Greece and the European Union (European Social Fund, ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning” in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (ΙΚΥ). We also acknowledge the editors of this volume for the helpful suggestions.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, Department of Biotechnology, Agricultural University of Athens, Athens, Greece

    Konstantinos Koudounas, Margarita Thomopoulou, Elisavet Angeli, Dikran Tsitsekian, Stamatis Rigas & Polydefkis Hatzopoulos

  2. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Konstantinos Koudounas

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  1. Konstantinos Koudounas
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  2. Margarita Thomopoulou
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  3. Elisavet Angeli
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  4. Dikran Tsitsekian
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  5. Stamatis Rigas
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  6. Polydefkis Hatzopoulos
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Correspondence to Konstantinos Koudounas or Polydefkis Hatzopoulos .

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  1. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Vincent Courdavault

  2. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Sébastien Besseau

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Koudounas, K., Thomopoulou, M., Angeli, E., Tsitsekian, D., Rigas, S., Hatzopoulos, P. (2020). Virus-Induced Gene Silencing in Olive Tree (Oleaceae). In: Courdavault, V., Besseau, S. (eds) Virus-Induced Gene Silencing in Plants. Methods in Molecular Biology, vol 2172. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0751-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0751-0_13

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