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Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model

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Basophils and Mast Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2163))

Abstract

The organotypic co-culture skin model has been providing an advanced approach to in vitro investigations of the skin. Mast cells, containing various mediators such as tryptase and chymase, are thought to contribute to many physiological and pathological events of the skin interactively with other cells. Here, we introduce an organotypic co-culture skin model which successfully integrates human dermal mast cells for further study of mast cell interactions with fibroblasts and keratinocytes.

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References

  1. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T (1981) Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science 211:1052–1054

    Article  CAS  PubMed  Google Scholar 

  2. Parenteau NL, Nolte CM, Bilbo P et al (1991) Epidermis generated in vitro: practical considerations and applications. J Cell Biochem 45:245–251

    Article  CAS  PubMed  Google Scholar 

  3. Fusenig NE (1994) Epithelial-mesenchymal interactions regulate keratinocyte growth and differentiation in vitro. In: Leigh IM, Lane EB, Watt FM (eds) The keratinocytes handbook. Cambridge University Press, Cambridge

    Google Scholar 

  4. Asselineau D, Bernard BA, Bailly D, Darmon M (1989) Retinoic acid improves epidermal morphogenesis. Dev Biol 133:322–335

    Article  CAS  PubMed  Google Scholar 

  5. Lenoir MC, Bernard BA (1990) Architecture of reconstructed epidermis on collagen lattices varies according to the method used: a comparative study. Skin Pharmacol 3:97–106

    Article  CAS  PubMed  Google Scholar 

  6. Parenteau NL, Bilbo P, Nolte CJ et al (1992) The organotypical culture of human skin keratinocytes and fibroblasts to achieve form and function. Cytotechnology 9:163–171

    Article  CAS  PubMed  Google Scholar 

  7. Igarashi M, Irwin CR, Locke M, Mackenzie IC (2003) Construction of large area organotypical cultures of oral mucosa and skin. J Oral Pathol Med 32:422–430

    Article  CAS  PubMed  Google Scholar 

  8. Maas-Szabowski N, Stark HJ, Fusenig NE (2000) Keratinocyte growth regulation in defined organotypic cultures through IL-1-induced keratinocyte growth factor expression in resting fibroblasts. J Invest Dermatol 114:1075–1084

    Article  CAS  PubMed  Google Scholar 

  9. Smola H, Stark HJ, Thiekotter G et al (1998) Dynamics of basement membrane formation by keratinocyte–fibroblast interactions in organotypic skin culture. Exp Cell Res 239:399–410

    Article  CAS  PubMed  Google Scholar 

  10. Marionnet C, Pierrard C, Vioux-Chagnoleau C et al (2006) Interactions between fibroblasts and keratinocytes in morphogenesis of dermal epidermal junction in a model of reconstructed skin. J Invest Dermatol 126:971–979

    Article  CAS  PubMed  Google Scholar 

  11. Ruoss SJ, Hartmann T, Caughey GH (1991) Mast cell tryptase is a mitogen for cultured fibroblasts. J Clin Invest 88:493–499

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Nadel JA (1991) Biology of mast cell tryptase and chymase. Ann N Y Acad Sci 629:319–331

    Article  CAS  PubMed  Google Scholar 

  13. Algermissen B, Hermes B, Feldmann-Böddeker I et al (1999) Mast cell chymase and tryptase during tissue turnover—analysis on in vitro mitogenesis of fibroblasts and keratinocytes and alterations in cutaneous scars. Exp Dermatol 8:193–198

    Article  CAS  PubMed  Google Scholar 

  14. Grabbe J, Welker P, Dippel E, Czarnetzki BM (1994) Stem cell factor, a novel cutaneous growth factor for mast cells and melanocytes. Arch Dermatol Res 287:78–84

    Article  CAS  PubMed  Google Scholar 

  15. Metcalfe DD, Baram D, Mekori YA (1997) Mast cells. Physiol Rev 77:1033–1079

    Article  CAS  PubMed  Google Scholar 

  16. Artuc M, Hermes B, Steckelings MU et al (1999) Mast cells and their mediators in wound healing—active participants or innocent bystanders? Exp Dermatol 8:1–16

    Article  CAS  PubMed  Google Scholar 

  17. Henz BM, Maurer M, Lippert U et al (2001) Mast cells as initiators of immunity and host defense. Exp Dermatol 10:1–10

    Article  CAS  PubMed  Google Scholar 

  18. Meng H, Marchese MJ, Garlick JA et al (1995) Mast cells induce T-cell adhesion to human fibroblasts by regulating intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression. J Invest Dermatol 105:789–796

    Article  CAS  PubMed  Google Scholar 

  19. Gruber BL, Kew RR, Jelask A et al (1997) Human mast cells activate fibroblasts. Tryptase is a fibrogenic factor stimulating collagen messenger ribonucleic acid synthesis and fibroblast chemotaxis. J Immunol 158:2310–2317

    Article  CAS  PubMed  Google Scholar 

  20. Gailit J, Marchese MJ, Kew RR, Gruber BL (2001) The differentiation and function of myofibroblasts is regulated by mast cell mediators. J Invest Dermatol 117:1113–1119

    Article  CAS  PubMed  Google Scholar 

  21. Artuc M, Steckelings UM, Grützkau A et al (2002) A long-term coculture model for the study of mast cell-keratinocyte interactions. J Invest Dermatol 119:411–415

    Article  CAS  PubMed  Google Scholar 

  22. Lawrence ID, Warner JA, Cohan VL et al (1987) Purification and characterization of human skin mast cells. Evidence for human mast cell heterogeneity. J Immunol 139:3062–3069

    Article  CAS  PubMed  Google Scholar 

  23. Grützkau A, Henz BM, Kirchhof L et al (2000) Alpha-melanocyte stimulating hormone acts as a selective inducer of secretory functions in human mast cells. Biochem Biophys Res Commun 278:14–19

    Article  PubMed  Google Scholar 

  24. Guhl S, Artuc M, Neou A et al (2011) Long-term cultured human skin mast cells are suitable for pharmacological studies of anti-allergic drugs due to high responsiveness to FcεRI cross-linking. Biosci Biotechnol Biochem 75:382–384

    Article  CAS  PubMed  Google Scholar 

  25. Normand J, Karasek MA (1995) A method for the isolation and serial propagation of keratinocytes, endothelial cells, and fibroblasts from a single punch biopsy of human skin. In Vitro Cell Dev Biol Anim 31:447–455

    Article  CAS  PubMed  Google Scholar 

  26. Stark HJ, Baur M, Breitkreutz D et al (1999) Organotypic keratinocyte cocultures in defined medium with regular epidermal morphogenesis and differentiation. J Invest Dermatol 112:681–691

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Metin Artuc .

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Kim, J., Guhl, S., Babina, M., Zuberbier, T., Artuc, M. (2020). Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model. In: Gibbs, B.F., Falcone, F.H. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 2163. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0696-4_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0696-4_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0695-7

  • Online ISBN: 978-1-0716-0696-4

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