Abstract
The three-dimensional culturing of human keratinocytes at the air–liquid interface yields a fully stratified epidermis including a functional stratum corneum and thus enables the study on epidermal structure and function in the context of biomedical, toxicological and pharmaceutical sciences. Here we provide a step-by-step detailed protocol for the isolation of human primary keratinocytes and the development of human epidermal equivalents generated from primary keratinocytes or immortalized keratinocytes (N/TERT-1; N/TERT-2G), including widely accepted procedures for the analysis of barrier function, tissue morphology, cell proliferation, and gene expression.
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Rikken, G., Niehues, H., van den Bogaard, E.H. (2020). Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines. In: Botchkareva, ​.V., Westgate, G.E. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 2154. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0648-3_5
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DOI: https://doi.org/10.1007/978-1-0716-0648-3_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0647-6
Online ISBN: 978-1-0716-0648-3
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